Abnormalities in cell proliferation and apico-basal cell polarity are separable in Drosophila lgl mutant clones in the developing eye

Nicola A. Grzeschik, Nancy Amin, Julie Secombe, Anthony M. Brumby, Helena E. Richardson

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

In homozygous mutants of Drosophila lethal-2-giant larvae (lgl), tissues lose apico-basal cell polarity and exhibit ectopic proliferation. Here, we use clonal analysis in the developing eye to investigate the effect of lgl null mutations in the context of surrounding wild-type tissue. lgl- clones in the larval eye disc exhibit ectopic expression of the G1-S regulator, Cyclin E, and ectopic proliferation, but do not lose apico-basal cell polarity. Decreasing the perdurance of Lgl protein in larval eye disc clones, by forcing extra proliferation of lgl- tissue (using a Minute background), leads to a loss in cell polarity and to more extreme ectopic cell proliferation. Later in development at the pupal stage, lgl mutant photoreceptor cells show aberrant apico-basal cell polarity, but this is not associated with ectopic proliferation, presumably because cells are differentiated. Thus in a clonal context, the ectopic proliferation and cell polarity defects of lgl- mutants are separable. Furthermore, lgl- mosaic eye discs have alterations in the normal patterns of apoptosis: in larval discs some lgl- and wild-type cells at the clonal boundary undergo apoptosis and are excluded from the epithelia, but apoptosis is decreased elsewhere in the disc, and in pupal retinas lgl- tissue shows less apoptosis.

Original languageEnglish (US)
Pages (from-to)106-123
Number of pages18
JournalDevelopmental Biology
Volume311
Issue number1
DOIs
StatePublished - Nov 1 2007
Externally publishedYes

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Cell Polarity
Drosophila
Larva
Clone Cells
Cell Proliferation
Apoptosis
Cyclin E
Photoreceptor Cells
Retina
Epithelium
Mutation

Keywords

  • Apico-basal cell polarity
  • Apoptosis
  • cyclin E
  • Drosophila
  • Eye development
  • lgl
  • Proliferation

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Abnormalities in cell proliferation and apico-basal cell polarity are separable in Drosophila lgl mutant clones in the developing eye. / Grzeschik, Nicola A.; Amin, Nancy; Secombe, Julie; Brumby, Anthony M.; Richardson, Helena E.

In: Developmental Biology, Vol. 311, No. 1, 01.11.2007, p. 106-123.

Research output: Contribution to journalArticle

Grzeschik, Nicola A. ; Amin, Nancy ; Secombe, Julie ; Brumby, Anthony M. ; Richardson, Helena E. / Abnormalities in cell proliferation and apico-basal cell polarity are separable in Drosophila lgl mutant clones in the developing eye. In: Developmental Biology. 2007 ; Vol. 311, No. 1. pp. 106-123.
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