TY - JOUR
T1 - A triclinic crystal form of the lectin concanavalin A
AU - Kanellopoulos, Panagiotis N.
AU - Tucker, Paul A.
AU - Pavlou, Kyriaki
AU - Agianian, Bogos
AU - Hamodrakas, Stavros J.
N1 - Funding Information:
S.J.H. thanks the Supercomputing Resource for Molecular Biology (SRMB), funded by the European Union ‘‘Access to Large Scale Installations’’ Programme, for support.
PY - 1996/7
Y1 - 1996/7
N2 - The molecular structure of a triclinic crystal form of concanavalin A has been refined at 2.4 Å resolution. The crystals have unit cell dimensions a = 78.8 Å, b = 79.3 Å, c = 133.3 Å, α = 97.1°, β = 90.2°, and γ = 97.5° and contain two tetramers per asymmetric unit each with approximate 222 symmetry. The final crystallographic R-factor is 0.205 and the free-R-factor is 0.265 in the resolution range 6.0 to 2.4 Å. The conformation of the tetramer is more similar to that found in concanavalin A saccharide complexes than in the previously reported I222 crystal form of uncomplexed concanavalin A. A comparison of the molecular packing between the two crystal forms shows a more open arrangement with large solvent channels through the crystal.
AB - The molecular structure of a triclinic crystal form of concanavalin A has been refined at 2.4 Å resolution. The crystals have unit cell dimensions a = 78.8 Å, b = 79.3 Å, c = 133.3 Å, α = 97.1°, β = 90.2°, and γ = 97.5° and contain two tetramers per asymmetric unit each with approximate 222 symmetry. The final crystallographic R-factor is 0.205 and the free-R-factor is 0.265 in the resolution range 6.0 to 2.4 Å. The conformation of the tetramer is more similar to that found in concanavalin A saccharide complexes than in the previously reported I222 crystal form of uncomplexed concanavalin A. A comparison of the molecular packing between the two crystal forms shows a more open arrangement with large solvent channels through the crystal.
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U2 - 10.1006/jsbi.1996.0065
DO - 10.1006/jsbi.1996.0065
M3 - Article
AN - SCOPUS:0030198976
SN - 1047-8477
VL - 117
SP - 16
EP - 23
JO - Journal of Structural Biology
JF - Journal of Structural Biology
IS - 1
ER -