A subset of host B lymphocytes controls melanoma metastasis through a melanoma cell adhesion molecule/MUC18-dependent interaction: Evidence from mice and humans

Fernanda I. Staquicini, Anita Tandle, Steven K. Libutti, Jessica Sun, Maya Zigler, Menashe Bar-Eli, Fabiana Aliperti, Elizabeth C. Pérez, Jeffrey E. Gershenwald, Mario Mariano, Renata Pasqualini, Wadih Arap, José Daniel Lopes

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Host immunity affects tumor metastasis but the corresponding cellular and molecular mechanisms are not entirely clear. Here, we show that a subset of B lymphocytes (termed B-1 population), but not other lymphocytes, has prometastatic effects on melanoma cells in vivo through a direct heterotypic cell-cell interaction. In the classic B16 mouse melanoma model, one mechanism underlying this phenomenon is a specific up-regulation and subsequent homophilic interaction mediated by the cell surface glycoprotein MUC18 (also known as melanoma cell adhesion molecule). Presence of B-1 lymphocytes in a panel of tumor samples from melanoma patients directly correlates with MUC18 expression in melanoma cells, indicating that the same protein interaction exists in humans. These results suggest a new but as yet unrecognized functional role for host B-1 lymphocytes in tumor metastasis and establish a biochemical basis for such observations. Our findings support the counterintuitive central hypothesis in which a primitive layer of the immune system actually contributes to tumor progression and metastasis in a mouse model and in melanoma patients. Given that monoclonal antibodies against MUC18 are in preclinical development but the reason for their antitumor activity is not well understood, these translational results are relevant in the setting of human melanoma and perhaps of other cancers.

Original languageEnglish (US)
Pages (from-to)8419-8428
Number of pages10
JournalCancer Research
Volume68
Issue number20
DOIs
StatePublished - Oct 15 2008
Externally publishedYes

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CD146 Antigens
B-Lymphocyte Subsets
Melanoma
Neoplasm Metastasis
Lymphocytes
Neoplasms
Experimental Melanomas
Membrane Glycoproteins
Cell Communication
Immune System
Immunity
Up-Regulation
Monoclonal Antibodies
Population

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

A subset of host B lymphocytes controls melanoma metastasis through a melanoma cell adhesion molecule/MUC18-dependent interaction : Evidence from mice and humans. / Staquicini, Fernanda I.; Tandle, Anita; Libutti, Steven K.; Sun, Jessica; Zigler, Maya; Bar-Eli, Menashe; Aliperti, Fabiana; Pérez, Elizabeth C.; Gershenwald, Jeffrey E.; Mariano, Mario; Pasqualini, Renata; Arap, Wadih; Lopes, José Daniel.

In: Cancer Research, Vol. 68, No. 20, 15.10.2008, p. 8419-8428.

Research output: Contribution to journalArticle

Staquicini, FI, Tandle, A, Libutti, SK, Sun, J, Zigler, M, Bar-Eli, M, Aliperti, F, Pérez, EC, Gershenwald, JE, Mariano, M, Pasqualini, R, Arap, W & Lopes, JD 2008, 'A subset of host B lymphocytes controls melanoma metastasis through a melanoma cell adhesion molecule/MUC18-dependent interaction: Evidence from mice and humans', Cancer Research, vol. 68, no. 20, pp. 8419-8428. https://doi.org/10.1158/0008-5472.CAN-08-1242
Staquicini, Fernanda I. ; Tandle, Anita ; Libutti, Steven K. ; Sun, Jessica ; Zigler, Maya ; Bar-Eli, Menashe ; Aliperti, Fabiana ; Pérez, Elizabeth C. ; Gershenwald, Jeffrey E. ; Mariano, Mario ; Pasqualini, Renata ; Arap, Wadih ; Lopes, José Daniel. / A subset of host B lymphocytes controls melanoma metastasis through a melanoma cell adhesion molecule/MUC18-dependent interaction : Evidence from mice and humans. In: Cancer Research. 2008 ; Vol. 68, No. 20. pp. 8419-8428.
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