A subpopulation of tegument protein vhs localizes to detergent-insoluble lipid rafts in herpes simplex virus-infected cells

Grace E. Lee, Geoffrey A. Church, Duncan W. Wilson

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Virion host shutoff (vhs) is a 58-kDa protein encoded by the UL41 gene of herpes simplex virus (HSV). vhs resides within the tegument of HSV, enters the cell cytoplasm at infection, and destabilizes host cell and viral mRNA. Late in infection, vhs must be assembled into the tegument of progeny virions, a poorly understood process. Using an anti-vhs antiserum and Western blotting of total cell or cytoplasmic extracts, we found that vhs is largely insoluble in HSV-infected cells, even in the presence of high levels of salt and the detergent Triton X-100. Furthermore, a subpopulation of vhs appears to be associated with detergent-insoluble lipid rafts and this raft population is enriched in a cytoplasmic fraction which contains assembling and mature HSV particles. Our data raise the possibility that HSV tegument polypeptides associate with membrane rafts, in common with the matrix proteins of a number of other viruses.

Original languageEnglish (US)
Pages (from-to)2038-2045
Number of pages8
JournalJournal of Virology
Volume77
Issue number3
DOIs
StatePublished - Feb 2003

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herpes simplex
Simplexvirus
virion
detergents
Virion
Detergents
Lipids
viruses
lipids
Proteins
proteins
cells
Octoxynol
Infection
infection
antiserum
Immune Sera
polypeptides
Cytoplasm
Western blotting

ASJC Scopus subject areas

  • Immunology

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A subpopulation of tegument protein vhs localizes to detergent-insoluble lipid rafts in herpes simplex virus-infected cells. / Lee, Grace E.; Church, Geoffrey A.; Wilson, Duncan W.

In: Journal of Virology, Vol. 77, No. 3, 02.2003, p. 2038-2045.

Research output: Contribution to journalArticle

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