A sphingolipid-dependent diffusion barrier confines ER stress to the yeast mother cell

Lori Clay, Fabrice Caudron, Annina Denoth-Lippuner, Barbara Boettcher, Stéphanie Buvelot Frei, Erik Lee Snapp, Yves Barral

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

In many cell types, lateral diffusion barriers compartmentalize the plasma membrane and, at least in budding yeast, the endoplasmic reticulum (ER). However, the molecular nature of these barriers, their mode of action and their cellular functions are unclear. Here, we show that misfolded proteins of the ER remain confined into the mother compartment of budding yeast cells. Confinement required the formation of a lateral diffusion barrier in the form of a distinct domain of the ER-membrane at the bud neck, in a septin-, Bud1 GTPase- and sphingolipid-dependent manner. The sphingolipids, but not Bud1, also contributed to barrier formation in the outer membrane of the dividing nucleus. Barrier-dependent confinement of ER stress into the mother cell promoted aging. Together, our data clarify the physical nature of lateral diffusion barriers in the ER and establish the role of such barriers in the asymmetric segregation of proteotoxic misfolded proteins during cell division and aging.

Original languageEnglish (US)
Article numbere01883
JournaleLife
Volume2014
Issue number3
DOIs
StatePublished - May 6 2014

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Sphingolipids
Endoplasmic Reticulum Stress
Diffusion barriers
Endoplasmic Reticulum
Yeast
Stem Cells
Yeasts
Saccharomycetales
Cell Aging
Aging of materials
Septins
Cells
Membranes
GTP Phosphohydrolases
Cell membranes
Proteins
Cell Division
Cell Membrane

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Medicine(all)
  • Neuroscience(all)

Cite this

Clay, L., Caudron, F., Denoth-Lippuner, A., Boettcher, B., Frei, S. B., Snapp, E. L., & Barral, Y. (2014). A sphingolipid-dependent diffusion barrier confines ER stress to the yeast mother cell. eLife, 2014(3), [e01883]. https://doi.org/10.7554/eLife.01883

A sphingolipid-dependent diffusion barrier confines ER stress to the yeast mother cell. / Clay, Lori; Caudron, Fabrice; Denoth-Lippuner, Annina; Boettcher, Barbara; Frei, Stéphanie Buvelot; Snapp, Erik Lee; Barral, Yves.

In: eLife, Vol. 2014, No. 3, e01883, 06.05.2014.

Research output: Contribution to journalArticle

Clay, L, Caudron, F, Denoth-Lippuner, A, Boettcher, B, Frei, SB, Snapp, EL & Barral, Y 2014, 'A sphingolipid-dependent diffusion barrier confines ER stress to the yeast mother cell', eLife, vol. 2014, no. 3, e01883. https://doi.org/10.7554/eLife.01883
Clay L, Caudron F, Denoth-Lippuner A, Boettcher B, Frei SB, Snapp EL et al. A sphingolipid-dependent diffusion barrier confines ER stress to the yeast mother cell. eLife. 2014 May 6;2014(3). e01883. https://doi.org/10.7554/eLife.01883
Clay, Lori ; Caudron, Fabrice ; Denoth-Lippuner, Annina ; Boettcher, Barbara ; Frei, Stéphanie Buvelot ; Snapp, Erik Lee ; Barral, Yves. / A sphingolipid-dependent diffusion barrier confines ER stress to the yeast mother cell. In: eLife. 2014 ; Vol. 2014, No. 3.
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