A specific monoclonal antibody (PG-B6) detects expression of the BCL-6 protein in germinal center B cells

L. Flenghi, B. Hilda Ye, M. Fizzotti, B. Bigerna, G. Cattoretti, S. Venturi, R. Pacini, S. Pileri, F. Lo Coco, E. Pescarmona, P. G. Pelicci, R. Dalla-Favera, B. Falini

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Abstract

The BCL-6 gene is frequently involved in translocations occurring at the 3q27 locus and is rearranged in approximately 30% of diffuse large cell lymphomas and in a small fraction of follicular lymphomas. The BCL-6 gene encodes for a Kruppel-type zinc-finger protein, the cell/tissue expression and function of which is unknown. In this study, we describe a new monoclonal antibody (PG-B6) that is specifically directed against a fixative-sensitive epitope on the amino-terminal region of the BCL-6 protein. By immunocytochemical analysis, BCL-6 localizes in the nucleus where PG-B6 staining gives a microgranular/diffuse pattern with exclusion of the nucleoli. The main reactivity of PG-B6 in tonsil and spleen is with the nuclei of germinal center B cells, whereas B cells within the mantle and marginal zones do not express BCL-6. No other lymphoid cells in the tonsil express BCL-6 except for a subset of CD3+/CD4+ intrafollicular and interfollicular T cells. A few lymphoid cells of unknown phenotype express BCL-6 in the thymus. Extra-lymphoid BCL-6 expression includes a weak nuclear positivity of epithelia. In non-Hodgkin's lymphomas, BCL-6 expression parallels that observed in normal lymphoid compartments, eg, expression in germinalcenter-derived tumors (follicular and diffuse large cell lymphomas), but not in mantle cell and marginal zone lymphomas. In most diffuse large cell lymphomas, the BCL-6 protein is expressed at high levels in cases with or without BCL-6 gene rearrangements. These findings indicate that BCL-6 expression is specifically regulated during B lymphocyte development and suggest that BCL-6 may play a role during B cell differentiation in the germinal center.

Original languageEnglish (US)
Pages (from-to)405-411
Number of pages7
JournalAmerican Journal of Pathology
Volume147
Issue number2
StatePublished - 1995
Externally publishedYes

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Germinal Center
Lymphoma, Large B-Cell, Diffuse
B-Lymphocytes
Monoclonal Antibodies
Follicular Lymphoma
Palatine Tonsil
Proteins
Lymphocytes
Fixatives
Gene Rearrangement
Zinc Fingers
Non-Hodgkin's Lymphoma
Thymus Gland
Genes
Epitopes
Cell Differentiation
Lymphoma
Spleen
Epithelium
Staining and Labeling

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Flenghi, L., Ye, B. H., Fizzotti, M., Bigerna, B., Cattoretti, G., Venturi, S., ... Falini, B. (1995). A specific monoclonal antibody (PG-B6) detects expression of the BCL-6 protein in germinal center B cells. American Journal of Pathology, 147(2), 405-411.

A specific monoclonal antibody (PG-B6) detects expression of the BCL-6 protein in germinal center B cells. / Flenghi, L.; Ye, B. Hilda; Fizzotti, M.; Bigerna, B.; Cattoretti, G.; Venturi, S.; Pacini, R.; Pileri, S.; Lo Coco, F.; Pescarmona, E.; Pelicci, P. G.; Dalla-Favera, R.; Falini, B.

In: American Journal of Pathology, Vol. 147, No. 2, 1995, p. 405-411.

Research output: Contribution to journalArticle

Flenghi, L, Ye, BH, Fizzotti, M, Bigerna, B, Cattoretti, G, Venturi, S, Pacini, R, Pileri, S, Lo Coco, F, Pescarmona, E, Pelicci, PG, Dalla-Favera, R & Falini, B 1995, 'A specific monoclonal antibody (PG-B6) detects expression of the BCL-6 protein in germinal center B cells', American Journal of Pathology, vol. 147, no. 2, pp. 405-411.
Flenghi, L. ; Ye, B. Hilda ; Fizzotti, M. ; Bigerna, B. ; Cattoretti, G. ; Venturi, S. ; Pacini, R. ; Pileri, S. ; Lo Coco, F. ; Pescarmona, E. ; Pelicci, P. G. ; Dalla-Favera, R. ; Falini, B. / A specific monoclonal antibody (PG-B6) detects expression of the BCL-6 protein in germinal center B cells. In: American Journal of Pathology. 1995 ; Vol. 147, No. 2. pp. 405-411.
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abstract = "The BCL-6 gene is frequently involved in translocations occurring at the 3q27 locus and is rearranged in approximately 30{\%} of diffuse large cell lymphomas and in a small fraction of follicular lymphomas. The BCL-6 gene encodes for a Kruppel-type zinc-finger protein, the cell/tissue expression and function of which is unknown. In this study, we describe a new monoclonal antibody (PG-B6) that is specifically directed against a fixative-sensitive epitope on the amino-terminal region of the BCL-6 protein. By immunocytochemical analysis, BCL-6 localizes in the nucleus where PG-B6 staining gives a microgranular/diffuse pattern with exclusion of the nucleoli. The main reactivity of PG-B6 in tonsil and spleen is with the nuclei of germinal center B cells, whereas B cells within the mantle and marginal zones do not express BCL-6. No other lymphoid cells in the tonsil express BCL-6 except for a subset of CD3+/CD4+ intrafollicular and interfollicular T cells. A few lymphoid cells of unknown phenotype express BCL-6 in the thymus. Extra-lymphoid BCL-6 expression includes a weak nuclear positivity of epithelia. In non-Hodgkin's lymphomas, BCL-6 expression parallels that observed in normal lymphoid compartments, eg, expression in germinalcenter-derived tumors (follicular and diffuse large cell lymphomas), but not in mantle cell and marginal zone lymphomas. In most diffuse large cell lymphomas, the BCL-6 protein is expressed at high levels in cases with or without BCL-6 gene rearrangements. These findings indicate that BCL-6 expression is specifically regulated during B lymphocyte development and suggest that BCL-6 may play a role during B cell differentiation in the germinal center.",
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T1 - A specific monoclonal antibody (PG-B6) detects expression of the BCL-6 protein in germinal center B cells

AU - Flenghi, L.

AU - Ye, B. Hilda

AU - Fizzotti, M.

AU - Bigerna, B.

AU - Cattoretti, G.

AU - Venturi, S.

AU - Pacini, R.

AU - Pileri, S.

AU - Lo Coco, F.

AU - Pescarmona, E.

AU - Pelicci, P. G.

AU - Dalla-Favera, R.

AU - Falini, B.

PY - 1995

Y1 - 1995

N2 - The BCL-6 gene is frequently involved in translocations occurring at the 3q27 locus and is rearranged in approximately 30% of diffuse large cell lymphomas and in a small fraction of follicular lymphomas. The BCL-6 gene encodes for a Kruppel-type zinc-finger protein, the cell/tissue expression and function of which is unknown. In this study, we describe a new monoclonal antibody (PG-B6) that is specifically directed against a fixative-sensitive epitope on the amino-terminal region of the BCL-6 protein. By immunocytochemical analysis, BCL-6 localizes in the nucleus where PG-B6 staining gives a microgranular/diffuse pattern with exclusion of the nucleoli. The main reactivity of PG-B6 in tonsil and spleen is with the nuclei of germinal center B cells, whereas B cells within the mantle and marginal zones do not express BCL-6. No other lymphoid cells in the tonsil express BCL-6 except for a subset of CD3+/CD4+ intrafollicular and interfollicular T cells. A few lymphoid cells of unknown phenotype express BCL-6 in the thymus. Extra-lymphoid BCL-6 expression includes a weak nuclear positivity of epithelia. In non-Hodgkin's lymphomas, BCL-6 expression parallels that observed in normal lymphoid compartments, eg, expression in germinalcenter-derived tumors (follicular and diffuse large cell lymphomas), but not in mantle cell and marginal zone lymphomas. In most diffuse large cell lymphomas, the BCL-6 protein is expressed at high levels in cases with or without BCL-6 gene rearrangements. These findings indicate that BCL-6 expression is specifically regulated during B lymphocyte development and suggest that BCL-6 may play a role during B cell differentiation in the germinal center.

AB - The BCL-6 gene is frequently involved in translocations occurring at the 3q27 locus and is rearranged in approximately 30% of diffuse large cell lymphomas and in a small fraction of follicular lymphomas. The BCL-6 gene encodes for a Kruppel-type zinc-finger protein, the cell/tissue expression and function of which is unknown. In this study, we describe a new monoclonal antibody (PG-B6) that is specifically directed against a fixative-sensitive epitope on the amino-terminal region of the BCL-6 protein. By immunocytochemical analysis, BCL-6 localizes in the nucleus where PG-B6 staining gives a microgranular/diffuse pattern with exclusion of the nucleoli. The main reactivity of PG-B6 in tonsil and spleen is with the nuclei of germinal center B cells, whereas B cells within the mantle and marginal zones do not express BCL-6. No other lymphoid cells in the tonsil express BCL-6 except for a subset of CD3+/CD4+ intrafollicular and interfollicular T cells. A few lymphoid cells of unknown phenotype express BCL-6 in the thymus. Extra-lymphoid BCL-6 expression includes a weak nuclear positivity of epithelia. In non-Hodgkin's lymphomas, BCL-6 expression parallels that observed in normal lymphoid compartments, eg, expression in germinalcenter-derived tumors (follicular and diffuse large cell lymphomas), but not in mantle cell and marginal zone lymphomas. In most diffuse large cell lymphomas, the BCL-6 protein is expressed at high levels in cases with or without BCL-6 gene rearrangements. These findings indicate that BCL-6 expression is specifically regulated during B lymphocyte development and suggest that BCL-6 may play a role during B cell differentiation in the germinal center.

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