A solution for stripping antibodies from polyvinylidene fluoride immunoblots for multiple reprobing

Yee Guide Yeung, E. Richard Stanley

Research output: Contribution to journalArticle

57 Scopus citations

Abstract

Available protocols for stripping antibodies from immunoblots involve the use of sodium dodecyl sulfate (SDS) or low-pH buffers. SDS was shown to remove transferred proteins from membranes, and low-pH buffer was shown to inefficiently strip off antibodies. A solution containing 6 M guanidine hydrochloride, 0.2% nondenaturing detergent, and a reducing agent can rapidly strip off tightly bound antibodies from aged polyvinylidene fluoride (PVDF) immunoblots at room temperature without removing significant amounts of transferred protein.

Original languageEnglish (US)
Pages (from-to)89-91
Number of pages3
JournalAnalytical Biochemistry
Volume389
Issue number1
DOIs
StatePublished - Jun 1 2009

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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