A role for molecular chaperone Hsc70 in reovirus outer capsid disassembly

Tijana Ivanovic, Melina A. Agosto, Kartik Chandran, Max L. Nibert

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

After crossing the cellular membrane barrier during cell entry, most animal viruses must undergo further disassembly before initiating viral gene expression. In many cases, these disassembly mechanisms remain poorly defined. For this report, we examined a final step in disassembly of the mammalian reovirus outer capsid: cytoplasmic release of the central, δfragment of membrane penetration protein μ1 to yield the transcriptionally active viral core particle. An in vitro assay with reticulocyte lysate recapitulated the release of intact δ molecules. Requirements for activity in this system were shown to include a protein factor, ATP, and Mg2+ and K + ions, consistent with involvement of a molecular chaperone such as Hsc70. Immunodepletion of Hsc70 and Hsp70 impaired δ release, which was then rescued by addition of purified Hsc70. Hsc70 was associated with released δ molecules not only in the lysate but also during cell entry. We conclude that Hsc70 plays a defined role in reovirus outer capsid disassembly, during or soon after membrane penetration, to prepare the entering particle for gene expression and replication.

Original languageEnglish (US)
Pages (from-to)12210-12219
Number of pages10
JournalJournal of Biological Chemistry
Volume282
Issue number16
DOIs
StatePublished - Apr 20 2007

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Molecular Chaperones
Capsid
Membranes
Gene Expression
Gene expression
Viral Genes
Reticulocytes
Virion
Membrane Proteins
Molecules
Adenosine Triphosphate
Ions
Viruses
Assays
Animals
Proteins
In Vitro Techniques

ASJC Scopus subject areas

  • Biochemistry

Cite this

A role for molecular chaperone Hsc70 in reovirus outer capsid disassembly. / Ivanovic, Tijana; Agosto, Melina A.; Chandran, Kartik; Nibert, Max L.

In: Journal of Biological Chemistry, Vol. 282, No. 16, 20.04.2007, p. 12210-12219.

Research output: Contribution to journalArticle

Ivanovic, Tijana ; Agosto, Melina A. ; Chandran, Kartik ; Nibert, Max L. / A role for molecular chaperone Hsc70 in reovirus outer capsid disassembly. In: Journal of Biological Chemistry. 2007 ; Vol. 282, No. 16. pp. 12210-12219.
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