A RhoC biosensor reveals differences in the activation kinetics of RhoA and RhoC in migrating cells

Jon S. Zawistowski, Mohsen Sabouri-Ghomi, Gaudenz Danuser, Klaus M. Hahn, Louis Hodgson

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

RhoA and RhoC GTPases share 92% amino acid sequence identity, yet play different roles in regulating cell motility and morphology. To understand these differences, we developed and validated a biosensor of RhoC activation (RhoC FLARE). This was used together with a RhoA biosensor to compare the spatio-temporal dynamics of RhoA and RhoC activity during cell protrusion/retraction and macropinocytosis. Both GTPases were activated similarly at the cell edge, but in regions more distal from the edge RhoC showed higher activation during protrusion. The two isoforms differed markedly in the kinetics of activation. RhoC was activated concomitantly with RhoA at the cell edge, but distally, RhoC activation preceded RhoA activation, occurring before edge protrusion. During macropinocytosis, differences were observed during vesicle closure and in the area surrounding vesicle formation.

Original languageEnglish (US)
Article numbere79877
JournalPloS one
Volume8
Issue number11
DOIs
StatePublished - Nov 5 2013

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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