A rapid ultra-performance liquid chromatography-electrospray ionisation mass spectrometric method for the analysis of saponins in the adventitious roots of Panax notoginseng

Mo Dan, Xie Guoxiang, Gao Xianfu, Long Xiangbao, Su Mingming, Zhao Aihua, Zhao Tie, Zhou Mingmei, Yunping Qiu, Jia Wei

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Introduction - Saponins are bioactive compounds employed in the prevention and treatment of cardiovascular and cerebrovascular diseases. The adventitious roots of Panax notoginseng may offer an alternative source of saponins. Identification and determination of saponins in the crude extract is challenging owing to their similar structures and the lack of standards. Objective - To develop a rapid, sensitive and accurate method based on solid-phase extraction followed by ultra-performance liquid chromatography-electrospray ionisation mass spectrometry (UPLC-ESI-MS) for the identification and quantification of saponins in P. notoginseng. Methodology - Following extraction using Waters Oasis™ HLB cartridges, the analytes were subjected to a UPLC system with a Waters Acquity BEH C18 chromatographic column and a binary mobile phase system consisting of 0.05% formic acid in water and acetonitrile under gradient elution conditions, with final detection by ESI-MS in the positive ion mode. Results - The UPLC-ESI-MS method gave limits of detection and quantification within the range 0.015-0.382 and 0.052-1.124 μg/mL, respectively, for 15 studied saponins. The instrumentation/injection precision (RSD) was 4.5% for a low concentration and 3.2% for an intermediate concentration sample. The intra- and inter-day repeatability was less than 2.65% (RSD). The method described was validated using spiked samples with different amounts of saponin standards. Conclusion - This UPLC-ESI-MS assay provides a suitable quality control method for the tentative identification and determination of major biological active constituents in adventitious and native roots of P. notoginseng.

Original languageEnglish (US)
Pages (from-to)68-76
Number of pages9
JournalPhytochemical Analysis
Volume20
Issue number1
DOIs
StatePublished - 2009
Externally publishedYes

Fingerprint

Panax notoginseng
ultra-performance liquid chromatography
Electrospray ionization
Saponins
Liquid chromatography
adventitious roots
saponins
Liquid Chromatography
ionization
Electrospray Ionization Mass Spectrometry
Mass spectrometry
formic acid
Water
methodology
cerebrovascular disorders
Cerebrovascular Disorders
oases
Solid Phase Extraction
instrumentation
solid phase extraction

Keywords

  • Adventitious root
  • Electrospray ionisation-mass spectrometry
  • Panax notoginseng
  • Solid-phase extraction
  • Ultra-performance liquid chromatography

ASJC Scopus subject areas

  • Complementary and alternative medicine
  • Molecular Medicine
  • Drug Discovery
  • Food Science
  • Biochemistry
  • Analytical Chemistry
  • Plant Science
  • Medicine(all)

Cite this

A rapid ultra-performance liquid chromatography-electrospray ionisation mass spectrometric method for the analysis of saponins in the adventitious roots of Panax notoginseng. / Dan, Mo; Guoxiang, Xie; Xianfu, Gao; Xiangbao, Long; Mingming, Su; Aihua, Zhao; Tie, Zhao; Mingmei, Zhou; Qiu, Yunping; Wei, Jia.

In: Phytochemical Analysis, Vol. 20, No. 1, 2009, p. 68-76.

Research output: Contribution to journalArticle

Dan, Mo ; Guoxiang, Xie ; Xianfu, Gao ; Xiangbao, Long ; Mingming, Su ; Aihua, Zhao ; Tie, Zhao ; Mingmei, Zhou ; Qiu, Yunping ; Wei, Jia. / A rapid ultra-performance liquid chromatography-electrospray ionisation mass spectrometric method for the analysis of saponins in the adventitious roots of Panax notoginseng. In: Phytochemical Analysis. 2009 ; Vol. 20, No. 1. pp. 68-76.
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abstract = "Introduction - Saponins are bioactive compounds employed in the prevention and treatment of cardiovascular and cerebrovascular diseases. The adventitious roots of Panax notoginseng may offer an alternative source of saponins. Identification and determination of saponins in the crude extract is challenging owing to their similar structures and the lack of standards. Objective - To develop a rapid, sensitive and accurate method based on solid-phase extraction followed by ultra-performance liquid chromatography-electrospray ionisation mass spectrometry (UPLC-ESI-MS) for the identification and quantification of saponins in P. notoginseng. Methodology - Following extraction using Waters Oasis™ HLB cartridges, the analytes were subjected to a UPLC system with a Waters Acquity BEH C18 chromatographic column and a binary mobile phase system consisting of 0.05{\%} formic acid in water and acetonitrile under gradient elution conditions, with final detection by ESI-MS in the positive ion mode. Results - The UPLC-ESI-MS method gave limits of detection and quantification within the range 0.015-0.382 and 0.052-1.124 μg/mL, respectively, for 15 studied saponins. The instrumentation/injection precision (RSD) was 4.5{\%} for a low concentration and 3.2{\%} for an intermediate concentration sample. The intra- and inter-day repeatability was less than 2.65{\%} (RSD). The method described was validated using spiked samples with different amounts of saponin standards. Conclusion - This UPLC-ESI-MS assay provides a suitable quality control method for the tentative identification and determination of major biological active constituents in adventitious and native roots of P. notoginseng.",
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T1 - A rapid ultra-performance liquid chromatography-electrospray ionisation mass spectrometric method for the analysis of saponins in the adventitious roots of Panax notoginseng

AU - Dan, Mo

AU - Guoxiang, Xie

AU - Xianfu, Gao

AU - Xiangbao, Long

AU - Mingming, Su

AU - Aihua, Zhao

AU - Tie, Zhao

AU - Mingmei, Zhou

AU - Qiu, Yunping

AU - Wei, Jia

PY - 2009

Y1 - 2009

N2 - Introduction - Saponins are bioactive compounds employed in the prevention and treatment of cardiovascular and cerebrovascular diseases. The adventitious roots of Panax notoginseng may offer an alternative source of saponins. Identification and determination of saponins in the crude extract is challenging owing to their similar structures and the lack of standards. Objective - To develop a rapid, sensitive and accurate method based on solid-phase extraction followed by ultra-performance liquid chromatography-electrospray ionisation mass spectrometry (UPLC-ESI-MS) for the identification and quantification of saponins in P. notoginseng. Methodology - Following extraction using Waters Oasis™ HLB cartridges, the analytes were subjected to a UPLC system with a Waters Acquity BEH C18 chromatographic column and a binary mobile phase system consisting of 0.05% formic acid in water and acetonitrile under gradient elution conditions, with final detection by ESI-MS in the positive ion mode. Results - The UPLC-ESI-MS method gave limits of detection and quantification within the range 0.015-0.382 and 0.052-1.124 μg/mL, respectively, for 15 studied saponins. The instrumentation/injection precision (RSD) was 4.5% for a low concentration and 3.2% for an intermediate concentration sample. The intra- and inter-day repeatability was less than 2.65% (RSD). The method described was validated using spiked samples with different amounts of saponin standards. Conclusion - This UPLC-ESI-MS assay provides a suitable quality control method for the tentative identification and determination of major biological active constituents in adventitious and native roots of P. notoginseng.

AB - Introduction - Saponins are bioactive compounds employed in the prevention and treatment of cardiovascular and cerebrovascular diseases. The adventitious roots of Panax notoginseng may offer an alternative source of saponins. Identification and determination of saponins in the crude extract is challenging owing to their similar structures and the lack of standards. Objective - To develop a rapid, sensitive and accurate method based on solid-phase extraction followed by ultra-performance liquid chromatography-electrospray ionisation mass spectrometry (UPLC-ESI-MS) for the identification and quantification of saponins in P. notoginseng. Methodology - Following extraction using Waters Oasis™ HLB cartridges, the analytes were subjected to a UPLC system with a Waters Acquity BEH C18 chromatographic column and a binary mobile phase system consisting of 0.05% formic acid in water and acetonitrile under gradient elution conditions, with final detection by ESI-MS in the positive ion mode. Results - The UPLC-ESI-MS method gave limits of detection and quantification within the range 0.015-0.382 and 0.052-1.124 μg/mL, respectively, for 15 studied saponins. The instrumentation/injection precision (RSD) was 4.5% for a low concentration and 3.2% for an intermediate concentration sample. The intra- and inter-day repeatability was less than 2.65% (RSD). The method described was validated using spiked samples with different amounts of saponin standards. Conclusion - This UPLC-ESI-MS assay provides a suitable quality control method for the tentative identification and determination of major biological active constituents in adventitious and native roots of P. notoginseng.

KW - Adventitious root

KW - Electrospray ionisation-mass spectrometry

KW - Panax notoginseng

KW - Solid-phase extraction

KW - Ultra-performance liquid chromatography

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