A proposed mechanism for the induction of cytotoxic T lymphocyte production by heat shock fusion proteins

Bryan K. Cho, Deborah Palliser, Eduardo Guillen, Jan Wisniewski, Richard A. Young, Jianzhu Chen, Herman N. Eisen

Research output: Contribution to journalArticle

127 Scopus citations

Abstract

A 65 kDa mycobacterial heat shock protein (hsp65), fused to a polypeptide that contains an octapeptide (SIYRYYGL) agonist for a particular T cell receptor (2C TCR), stimulated C57BL/6 mice as well as CD4-deficient mice to produce CD8+ cytolytic T lymphocytes (CTL) to the fusion partner's octapeptide. This and other hsp65 fusion proteins but not native hsp65 itself stimulated dendritic cells in vitro and in vivo to upregulate the levels of MHC (class I and II) and costimulatory (B7.2) molecules. The results suggest a mechanism for the general finding that hsp fusion proteins, having fusion partners of widely differing lengths and sequences, elicit CD8 CTL to peptides from the fusion partners without requiring exogenous adjuvants or the participation of CD4+ T cells.

Original languageEnglish (US)
Pages (from-to)263-272
Number of pages10
JournalImmunity
Volume12
Issue number3
DOIs
StatePublished - Jan 1 2000

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

Fingerprint Dive into the research topics of 'A proposed mechanism for the induction of cytotoxic T lymphocyte production by heat shock fusion proteins'. Together they form a unique fingerprint.

  • Cite this

    Cho, B. K., Palliser, D., Guillen, E., Wisniewski, J., Young, R. A., Chen, J., & Eisen, H. N. (2000). A proposed mechanism for the induction of cytotoxic T lymphocyte production by heat shock fusion proteins. Immunity, 12(3), 263-272. https://doi.org/10.1016/S1074-7613(00)80179-X