A proposed mechanism for the induction of cytotoxic T lymphocyte production by heat shock fusion proteins

Bryan K. Cho, Deborah Palliser, Eduardo Guillen, Jan Wisniewski, Richard A. Young, Jianzhu Chen, Herman N. Eisen

Research output: Contribution to journalArticle

127 Citations (Scopus)

Abstract

A 65 kDa mycobacterial heat shock protein (hsp65), fused to a polypeptide that contains an octapeptide (SIYRYYGL) agonist for a particular T cell receptor (2C TCR), stimulated C57BL/6 mice as well as CD4-deficient mice to produce CD8+ cytolytic T lymphocytes (CTL) to the fusion partner's octapeptide. This and other hsp65 fusion proteins but not native hsp65 itself stimulated dendritic cells in vitro and in vivo to upregulate the levels of MHC (class I and II) and costimulatory (B7.2) molecules. The results suggest a mechanism for the general finding that hsp fusion proteins, having fusion partners of widely differing lengths and sequences, elicit CD8 CTL to peptides from the fusion partners without requiring exogenous adjuvants or the participation of CD4+ T cells.

Original languageEnglish (US)
Pages (from-to)263-272
Number of pages10
JournalImmunity
Volume12
Issue number3
StatePublished - 2000
Externally publishedYes

Fingerprint

Cytotoxic T-Lymphocytes
Heat-Shock Proteins
T-Lymphocytes
Peptides
T-Cell Antigen Receptor
Inbred C57BL Mouse
Dendritic Cells
Proteins
Up-Regulation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases
  • Immunology

Cite this

Cho, B. K., Palliser, D., Guillen, E., Wisniewski, J., Young, R. A., Chen, J., & Eisen, H. N. (2000). A proposed mechanism for the induction of cytotoxic T lymphocyte production by heat shock fusion proteins. Immunity, 12(3), 263-272.

A proposed mechanism for the induction of cytotoxic T lymphocyte production by heat shock fusion proteins. / Cho, Bryan K.; Palliser, Deborah; Guillen, Eduardo; Wisniewski, Jan; Young, Richard A.; Chen, Jianzhu; Eisen, Herman N.

In: Immunity, Vol. 12, No. 3, 2000, p. 263-272.

Research output: Contribution to journalArticle

Cho, BK, Palliser, D, Guillen, E, Wisniewski, J, Young, RA, Chen, J & Eisen, HN 2000, 'A proposed mechanism for the induction of cytotoxic T lymphocyte production by heat shock fusion proteins', Immunity, vol. 12, no. 3, pp. 263-272.
Cho BK, Palliser D, Guillen E, Wisniewski J, Young RA, Chen J et al. A proposed mechanism for the induction of cytotoxic T lymphocyte production by heat shock fusion proteins. Immunity. 2000;12(3):263-272.
Cho, Bryan K. ; Palliser, Deborah ; Guillen, Eduardo ; Wisniewski, Jan ; Young, Richard A. ; Chen, Jianzhu ; Eisen, Herman N. / A proposed mechanism for the induction of cytotoxic T lymphocyte production by heat shock fusion proteins. In: Immunity. 2000 ; Vol. 12, No. 3. pp. 263-272.
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