@article{02054d8ca16649dabb786d30b99fd639,
title = "A proposed mechanism for the induction of cytotoxic T lymphocyte production by heat shock fusion proteins",
abstract = "A 65 kDa mycobacterial heat shock protein (hsp65), fused to a polypeptide that contains an octapeptide (SIYRYYGL) agonist for a particular T cell receptor (2C TCR), stimulated C57BL/6 mice as well as CD4-deficient mice to produce CD8+ cytolytic T lymphocytes (CTL) to the fusion partner's octapeptide. This and other hsp65 fusion proteins but not native hsp65 itself stimulated dendritic cells in vitro and in vivo to upregulate the levels of MHC (class I and II) and costimulatory (B7.2) molecules. The results suggest a mechanism for the general finding that hsp fusion proteins, having fusion partners of widely differing lengths and sequences, elicit CD8 CTL to peptides from the fusion partners without requiring exogenous adjuvants or the participation of CD4+ T cells.",
author = "Cho, {Bryan K.} and Deborah Palliser and Eduardo Guillen and Jan Wisniewski and Young, {Richard A.} and Jianzhu Chen and Eisen, {Herman N.}",
note = "Funding Information: We are grateful to Carol McKinley for skillful technical help; Nir Hacohen for advice about dendritic cells; Glenn Paradis for help with flow cytometry; Richard Cook of the MIT biopolymer laboratory for peptide synthesis, amino acid analyses, and mass spectrometry; Evelyn Wiebe for endotoxin assays; and Jun Liu and Satoshi Sugawa for helpful discussions. We are grateful to Vical for the VR1055 plasmid. B. K. C. was supported by a National Institutes of Health training grant (5T32-AI-07463) and D. P. by a Stressgen postdoctoral fellowship. This work was supported in part by a National Institutes of Health Cancer Center core grant (CA-14051) and National Institutes of Health research grants (CA-60686, AI-44477, and AI-44478).",
year = "2000",
doi = "10.1016/S1074-7613(00)80179-X",
language = "English (US)",
volume = "12",
pages = "263--272",
journal = "Immunity",
issn = "1074-7613",
publisher = "Cell Press",
number = "3",
}