TY - JOUR
T1 - A portable microkeratome for harvesting the human anterior corneal surface
AU - Sarayba, Melvin A.
AU - Tungsiripat, Tulaya
AU - Sweet, Paula M.
AU - Chuck, Roy S.
PY - 2004/7
Y1 - 2004/7
N2 - A portable, gas-driven turbine microkeratome device capable of harvesting the entire anterior corneal surface for lamellar transplantation on human donor globes was evaluated. The device consisted of a modified LASIK microkeratome with an enlarged suction ring, head, and blade. Vacuum was achieved by a simple hand pump. Lamellar keratectomy was performed on 5 fresh human donor globes. Lenticule dimensions were measured on days 0, 3, 6, and 9 after storage in preservation media at 4°C. On day 0, the obtained lenticules were 13.9 ± 0.9 mm and 13.5 ± 0.4 mm, vertical and horizontal diameters, respectively. The average central lenticule thickness was 152.2 ± 52 μm. Each lenticule was uniform in thickness over 5 measurement points (P = 0.74). Repeat measurements of corneal thickness over the 9 days showed no statistically significant difference (P = 0.51). On day 9 lenticules were 14.6 ± 0.3 mm and 14.6 ± 0.4 mm, vertical and horizontal diameters, respectively. When day 0 was compared to day 9, vertical diameter also showed no statistically significant difference (P = 0.16), whereas horizontal diameter was significantly different (P < 0.001). This device proves to be an economical alternative to electric-powered systems for the harvest of transplantable corneal sections.
AB - A portable, gas-driven turbine microkeratome device capable of harvesting the entire anterior corneal surface for lamellar transplantation on human donor globes was evaluated. The device consisted of a modified LASIK microkeratome with an enlarged suction ring, head, and blade. Vacuum was achieved by a simple hand pump. Lamellar keratectomy was performed on 5 fresh human donor globes. Lenticule dimensions were measured on days 0, 3, 6, and 9 after storage in preservation media at 4°C. On day 0, the obtained lenticules were 13.9 ± 0.9 mm and 13.5 ± 0.4 mm, vertical and horizontal diameters, respectively. The average central lenticule thickness was 152.2 ± 52 μm. Each lenticule was uniform in thickness over 5 measurement points (P = 0.74). Repeat measurements of corneal thickness over the 9 days showed no statistically significant difference (P = 0.51). On day 9 lenticules were 14.6 ± 0.3 mm and 14.6 ± 0.4 mm, vertical and horizontal diameters, respectively. When day 0 was compared to day 9, vertical diameter also showed no statistically significant difference (P = 0.16), whereas horizontal diameter was significantly different (P < 0.001). This device proves to be an economical alternative to electric-powered systems for the harvest of transplantable corneal sections.
KW - Anterior corneal surface
KW - Donor corneas
KW - Microkeratome
UR - http://www.scopus.com/inward/record.url?scp=3042784589&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=3042784589&partnerID=8YFLogxK
U2 - 10.1097/01.ico.0000122701.49054.c9
DO - 10.1097/01.ico.0000122701.49054.c9
M3 - Article
C2 - 15220727
AN - SCOPUS:3042784589
SN - 0277-3740
VL - 23
SP - 443
EP - 446
JO - Cornea
JF - Cornea
IS - 5
ER -