A pathogenesis-based transcript signature in donor-specific antibody-positive kidney transplant patients with normal biopsies

P. O. Broin, Nicole A.M. Hayde, Y. Bao, B. Ye, R. B. Calder, G. de Boccardo, M. Lubetzky, Maria Ajaimy, James M. Pullman, A. Colovai, Enver Akalin, A. Golden

Research output: Contribution to journalArticle

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Abstract

Affymetrix Human Gene 1.0-ST arrays were used to assess the gene expression profiles of kidney transplant patients who presented with donor-specific antibodies (DSAs) but showed normal biopsy histopathology and did not develop antibody-mediated rejection (AMR). Biopsy and whole-blood profiles for these DSA-positive, AMR-negative (DSA. +/AMR-) patients were compared to both DSA-positive, AMR-positive (DSA. +/AMR. +) patients as well as DSA-negative (DSA. -) controls. While individual gene expression changes across sample groups were relatively subtle, gene-set enrichment analysis using previously identified pathogenesis-based transcripts (PBTs) identified a clear molecular signature involving increased rejection-associated transcripts in AMR. - patients. Results from this study have been published in Kidney International (Hayde et al., 2014 [1]) and the associated data have been deposited in the GEO archive and are accessible via the following link: http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE50084.

Original languageEnglish (US)
Pages (from-to)357-360
Number of pages4
JournalGenomics Data
Volume2
DOIs
StatePublished - 2014

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Transplants
Biopsy
Tissue Donors
Kidney
Antibodies
Gene expression
Genes
Blood Donors
Transcriptome
Blood

Keywords

  • Antibody-mediated rejection
  • Donor-specific antibodies
  • Gene expression
  • Kidney
  • Transplant

ASJC Scopus subject areas

  • Molecular Medicine
  • Biochemistry
  • Biotechnology
  • Genetics

Cite this

A pathogenesis-based transcript signature in donor-specific antibody-positive kidney transplant patients with normal biopsies. / Broin, P. O.; Hayde, Nicole A.M.; Bao, Y.; Ye, B.; Calder, R. B.; de Boccardo, G.; Lubetzky, M.; Ajaimy, Maria; Pullman, James M.; Colovai, A.; Akalin, Enver; Golden, A.

In: Genomics Data, Vol. 2, 2014, p. 357-360.

Research output: Contribution to journalArticle

Broin, P. O. ; Hayde, Nicole A.M. ; Bao, Y. ; Ye, B. ; Calder, R. B. ; de Boccardo, G. ; Lubetzky, M. ; Ajaimy, Maria ; Pullman, James M. ; Colovai, A. ; Akalin, Enver ; Golden, A. / A pathogenesis-based transcript signature in donor-specific antibody-positive kidney transplant patients with normal biopsies. In: Genomics Data. 2014 ; Vol. 2. pp. 357-360.
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