A pantothenate auxotroph of Mycobacterium tuberculosis is highly attenuated and protects mice against tuberculosis

Vasan K. Sambandamurthy, Xiaojuan Wang, Bing Chen, Robert G. Russell, Steven Derrick, Frank M. Collins, Sheldon L. Morris, William R. Jacobs

Research output: Contribution to journalArticle

233 Citations (Scopus)

Abstract

With the advent of HIV and the widespread emergence of drugresistant strains of Mycobacterium tuberculosis, newer control strategies in the form of a better vaccine could decrease the global incidence of tuberculosis. A desirable trait in an effective live attenuated vaccine strain is an ability to persist within the host in a limited fashion in order to produce important protective antigens in vivo1,2. Attenuated M. tuberculosis vaccine candidates have been constructed by deleting genes required for growth in mice3-s. These candidate vaccines did not elicit adequate protective immunity in animal models, due to their inability to persist sufficiently long within the host tissues. Here we report that an auxotrophic mutant of M. tuberculosis defective in the de novo biosynthesis of pantothenic acid (vitamin B5) is highly attenuated in immunocompromised SCID mice and in immunocompetent BALB/c mice. SCID mice infected with the pantothenate auxotroph survived significantly longer (250 days) than mice infected with either bacille Calmette-Guerin (BCG) vaccine or virulent M. tuberculosis (77 and 35 days, respectively). Subcutaneous immunization with this auxotroph conferred protection in C57BL/6J mice against an aerosol challenge with virulent M. tuberculosis, which was comparable with that afforded by BCG vaccination. Our findings highlight the importance of de novo pantothenate biosynthesis in limiting the intracellular survival and pathogenesis of M. tuberculosis without reducing its immunogenicity in vaccinated mice.

Original languageEnglish (US)
Pages (from-to)1171-1174
Number of pages4
JournalNature Medicine
Volume8
Issue number10
DOIs
StatePublished - Oct 1 2002

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Mycobacterium tuberculosis
Pantothenic Acid
Tuberculosis
Vaccines
Biosynthesis
SCID Mice
Tuberculosis Vaccines
Immunization
Attenuated Vaccines
Aerosols
BCG Vaccine
Animals
Mycobacterium
Genes
Inbred C57BL Mouse
Tissue
Antigens
Immunity
Vaccination
Animal Models

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

A pantothenate auxotroph of Mycobacterium tuberculosis is highly attenuated and protects mice against tuberculosis. / Sambandamurthy, Vasan K.; Wang, Xiaojuan; Chen, Bing; Russell, Robert G.; Derrick, Steven; Collins, Frank M.; Morris, Sheldon L.; Jacobs, William R.

In: Nature Medicine, Vol. 8, No. 10, 01.10.2002, p. 1171-1174.

Research output: Contribution to journalArticle

Sambandamurthy, VK, Wang, X, Chen, B, Russell, RG, Derrick, S, Collins, FM, Morris, SL & Jacobs, WR 2002, 'A pantothenate auxotroph of Mycobacterium tuberculosis is highly attenuated and protects mice against tuberculosis', Nature Medicine, vol. 8, no. 10, pp. 1171-1174. https://doi.org/10.1038/nm765
Sambandamurthy, Vasan K. ; Wang, Xiaojuan ; Chen, Bing ; Russell, Robert G. ; Derrick, Steven ; Collins, Frank M. ; Morris, Sheldon L. ; Jacobs, William R. / A pantothenate auxotroph of Mycobacterium tuberculosis is highly attenuated and protects mice against tuberculosis. In: Nature Medicine. 2002 ; Vol. 8, No. 10. pp. 1171-1174.
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