A novel glycolipid antigen for NKT cells that preferentially induces IFN-γ production

Alysia M. Birkholz, Enrico Girardi, Gerhard Wingender, Archana Khurana, Jing Wang, Meng Zhao, Sonja Zahner, Petr A. Illarionov, Xiangshu Wen, Michelle Li, Weiming Yuan, Steven A. Porcelli, Gurdyal S. Besra, Dirk M. Zajonc, Mitchell Kronenberg

Research output: Contribution to journalArticle

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Abstract

In this article, we characterize a novel Ag for invariant NKT (iNKT) cells capable of producing an especially robust Th1 response. This glycosphingolipid, DB06-1, is similar in chemical structure to the well-studied a-galactosylceramide (aGalCer), with the only change being a single atom: the substitution of a carbonyl oxygen with a sulfur atom. Although DB06-1 is not a more effective Ag in vitro, the small chemical change has a marked impact on the ability of this lipid Ag to stimulate iNKT cells in vivo, with increased IFN-γ production at 24 h compared with αGalCer, increased IL-12, and increased activation of NK cells to produce IFN-γ. These changes are correlated with an enhanced ability of DB06-1 to load in the CD1d molecules expressed by dendritic cells in vivo. Moreover, structural studies suggest a tighter fit into the CD1d binding groove by DB06-1 compared with aGalCer. Surprisingly, when iNKT cells previously exposed to DB06-1 are restimulated weeks later, they have greatly increased IL-10 production. Therefore, our data are consistent with a model whereby augmented and or prolonged presentation of a glycolipid Ag leads to increased activation of NK cells and a Th1-skewed immune response, which may result, in part, from enhanced loading into CD1d. Furthermore, our data suggest that strong antigenic stimulation in vivo may lead to the expansion of IL-10-producing iNKT cells, which could counteract the benefits of increased early IFN-γ production.

Original languageEnglish (US)
Pages (from-to)924-933
Number of pages10
JournalJournal of Immunology
Volume195
Issue number3
DOIs
StatePublished - Aug 1 2015

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Natural Killer T-Cells
Glycolipids
Galactosylceramides
Antigens
Natural Killer Cells
Interleukin-10
CD1d Antigen
Glycosphingolipids
Interleukin-12
Sulfur
Dendritic Cells
Oxygen
Lipids

ASJC Scopus subject areas

  • Immunology

Cite this

Birkholz, A. M., Girardi, E., Wingender, G., Khurana, A., Wang, J., Zhao, M., ... Kronenberg, M. (2015). A novel glycolipid antigen for NKT cells that preferentially induces IFN-γ production. Journal of Immunology, 195(3), 924-933. https://doi.org/10.4049/jimmunol.1500070

A novel glycolipid antigen for NKT cells that preferentially induces IFN-γ production. / Birkholz, Alysia M.; Girardi, Enrico; Wingender, Gerhard; Khurana, Archana; Wang, Jing; Zhao, Meng; Zahner, Sonja; Illarionov, Petr A.; Wen, Xiangshu; Li, Michelle; Yuan, Weiming; Porcelli, Steven A.; Besra, Gurdyal S.; Zajonc, Dirk M.; Kronenberg, Mitchell.

In: Journal of Immunology, Vol. 195, No. 3, 01.08.2015, p. 924-933.

Research output: Contribution to journalArticle

Birkholz, AM, Girardi, E, Wingender, G, Khurana, A, Wang, J, Zhao, M, Zahner, S, Illarionov, PA, Wen, X, Li, M, Yuan, W, Porcelli, SA, Besra, GS, Zajonc, DM & Kronenberg, M 2015, 'A novel glycolipid antigen for NKT cells that preferentially induces IFN-γ production', Journal of Immunology, vol. 195, no. 3, pp. 924-933. https://doi.org/10.4049/jimmunol.1500070
Birkholz AM, Girardi E, Wingender G, Khurana A, Wang J, Zhao M et al. A novel glycolipid antigen for NKT cells that preferentially induces IFN-γ production. Journal of Immunology. 2015 Aug 1;195(3):924-933. https://doi.org/10.4049/jimmunol.1500070
Birkholz, Alysia M. ; Girardi, Enrico ; Wingender, Gerhard ; Khurana, Archana ; Wang, Jing ; Zhao, Meng ; Zahner, Sonja ; Illarionov, Petr A. ; Wen, Xiangshu ; Li, Michelle ; Yuan, Weiming ; Porcelli, Steven A. ; Besra, Gurdyal S. ; Zajonc, Dirk M. ; Kronenberg, Mitchell. / A novel glycolipid antigen for NKT cells that preferentially induces IFN-γ production. In: Journal of Immunology. 2015 ; Vol. 195, No. 3. pp. 924-933.
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AU - Wen, Xiangshu

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AU - Yuan, Weiming

AU - Porcelli, Steven A.

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