A nitrogen response pathway regulates virulence functions in Fusarium oxysporum via the protein kinase TOR and the bZIP protein MeaB

Manuel S. López-Berges, Nicolas Rispail, Rafael C. Prados-Rosales, Antoniodi di Pietro

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

During infection, fungal pathogens activate virulence mechanisms, such as host adhesion, penetration and invasive growth. In the vascular wilt fungus Fusarium oxysporum, the mitogen-activated protein kinase Fmk1 is required for plant infection and controls processes such as cellophane penetration, vegetative hyphal fusion, or root adhesion. Here, we show that these virulence-related functions are repressed by the preferred nitrogen source ammonium and restored by treatment with L-methionine sulfoximine or rapamycin, two specific inhibitors of Gln synthetase and the protein kinase TOR, respectively. Deletion of the bZIP protein MeaB also resulted in nitrogen source-independent activation of virulence mechanisms. Activation of these functions did not require the global nitrogen regulator AreA, suggesting that MeaB-mediated repression of virulence functions does not act through inhibition of AreA. Tomato plants (Solanum lycopersicum) supplied with ammonium rather than nitrate showed a significant reduction in vascular wilt symptoms when infected with the wild type but not with the DmeaB strain. Nitrogen source also affected invasive growth in the rice blast fungus Magnaporthe oryzae and the wheat head blight pathogen Fusarium graminearum. We propose that a conserved nitrogen-responsive pathway might operate via TOR and MeaB to control virulence in plant pathogenic fungi.

Original languageEnglish (US)
Pages (from-to)2459-2475
Number of pages17
JournalPlant Cell
Volume22
Issue number7
DOIs
StatePublished - Jul 2010
Externally publishedYes

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Basic-Leucine Zipper Transcription Factors
Fusarium
Fusarium oxysporum
protein kinases
Protein Kinases
Virulence
Nitrogen
virulence
vascular wilt
nitrogen
Fungi
Lycopersicon esculentum
Blood Vessels
adhesion
Methionine Sulfoximine
Magnaporthe
Cellophane
head blight
cellophane
Magnaporthe oryzae

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

A nitrogen response pathway regulates virulence functions in Fusarium oxysporum via the protein kinase TOR and the bZIP protein MeaB. / López-Berges, Manuel S.; Rispail, Nicolas; Prados-Rosales, Rafael C.; di Pietro, Antoniodi.

In: Plant Cell, Vol. 22, No. 7, 07.2010, p. 2459-2475.

Research output: Contribution to journalArticle

López-Berges, Manuel S. ; Rispail, Nicolas ; Prados-Rosales, Rafael C. ; di Pietro, Antoniodi. / A nitrogen response pathway regulates virulence functions in Fusarium oxysporum via the protein kinase TOR and the bZIP protein MeaB. In: Plant Cell. 2010 ; Vol. 22, No. 7. pp. 2459-2475.
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