A new Augmin subunit, Msd1, demonstrates the importance of mitotic spindle-templated microtubule nucleation in the absence of functioning centrosomes

Alan Wainman, Daniel W. Buster, Tommy Duncan, Jeremy Metz, Ao Ma, David J. Sharp, James G. Wakefield

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The Drosophila Augmin complex localizes γ-tubulin to the microtubules of the mitotic spindle, regulating the density of spindle microtubules in tissue culture cells. Here, we identify the microtubule-associated protein Msd1 as a new component of the Augmin complex and demonstrate directly that it is required for nucleation of microtubules from within the mitotic spindle. Although Msd1 is necessary for embryonic syncytial mitoses, flies possessing a mutation in msd1 are viable. Importantly, however, in the absence of centrosomes, microtubule nucleation from within the spindle becomes essential. Thus, the Augmin complex has a crucial role in the development of the fly.

Original languageEnglish (US)
Pages (from-to)1876-1881
Number of pages6
JournalGenes and Development
Volume23
Issue number16
DOIs
StatePublished - 2009

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Centrosome
Spindle Apparatus
Microtubules
Diptera
Microtubule-Associated Proteins
Tubulin
Mitosis
Drosophila
Cell Culture Techniques
Mutation

Keywords

  • γ-tubulin
  • Augmin
  • Drosophila
  • Msd1

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Medicine(all)

Cite this

A new Augmin subunit, Msd1, demonstrates the importance of mitotic spindle-templated microtubule nucleation in the absence of functioning centrosomes. / Wainman, Alan; Buster, Daniel W.; Duncan, Tommy; Metz, Jeremy; Ma, Ao; Sharp, David J.; Wakefield, James G.

In: Genes and Development, Vol. 23, No. 16, 2009, p. 1876-1881.

Research output: Contribution to journalArticle

Wainman, Alan ; Buster, Daniel W. ; Duncan, Tommy ; Metz, Jeremy ; Ma, Ao ; Sharp, David J. ; Wakefield, James G. / A new Augmin subunit, Msd1, demonstrates the importance of mitotic spindle-templated microtubule nucleation in the absence of functioning centrosomes. In: Genes and Development. 2009 ; Vol. 23, No. 16. pp. 1876-1881.
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