Abstract
Ischemic cardiomyopathy poses a significant public health burden due to the irreversible loss of functional cardiac tissue. Alternative treatment strategies include creation of three-dimensional (3D) cardiac tissues to both replace and augment injured native tissue. In this study, we utilize a net mold-based method to create a biomaterial-free 3D cardiac tissue and compare it to current methods using biomaterials. Cardiomyocytes, fibroblasts, and endothelial cells were combined using a hanging drop method to create spheroids. For the net mold patch method, spheroids were seeded into a net mold-based system to create biomaterial-free 3D cardiac patches. For the gel patch, spheroids were embedded in a collagen gel. Immunohistochemistry revealed increased alignment, vascularization, collagen I expression, cell viability, and higher density of cells in the net mold patch compared with the gel patch. Furthermore, in vivo testing in a left anterior descending artery ligation rat model found increased ejection fraction and smaller scar area following implantation of the net mold patch. We present a novel and simple reproducible method to create biomaterial-free 3D net mold patches that may potentially improve the treatment of heart failure in the future.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 243-252 |
| Number of pages | 10 |
| Journal | Tissue Engineering - Part C: Methods |
| Volume | 25 |
| Issue number | 4 |
| DOIs | |
| State | Published - Apr 1 2019 |
| Externally published | Yes |
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Keywords
- cardiac tissue engineering
- hanging drop spheroids
- heart failure
- mold-based method
ASJC Scopus subject areas
- Bioengineering
- Medicine (miscellaneous)
- Biomedical Engineering
Cite this
A Net Mold-Based Method of Biomaterial-Free Three-Dimensional Cardiac Tissue Creation. / Yang, Bai; Lui, Cecillia; Yeung, Enoch; Matsushita, Hiroshi; Jeyaram, Anjana; Pitaktong, Isaree; Inoue, Takahiro; Mohamed, Zayneb; Ong, Chin Siang; Disilvestre, Deborah; Jay, Steven M.; Tung, Leslie; Tomaselli, Gordon F.; Ma, Chunye; Hibino, Narutoshi.
In: Tissue Engineering - Part C: Methods, Vol. 25, No. 4, 01.04.2019, p. 243-252.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A Net Mold-Based Method of Biomaterial-Free Three-Dimensional Cardiac Tissue Creation
AU - Yang, Bai
AU - Lui, Cecillia
AU - Yeung, Enoch
AU - Matsushita, Hiroshi
AU - Jeyaram, Anjana
AU - Pitaktong, Isaree
AU - Inoue, Takahiro
AU - Mohamed, Zayneb
AU - Ong, Chin Siang
AU - Disilvestre, Deborah
AU - Jay, Steven M.
AU - Tung, Leslie
AU - Tomaselli, Gordon F.
AU - Ma, Chunye
AU - Hibino, Narutoshi
PY - 2019/4/1
Y1 - 2019/4/1
N2 - Ischemic cardiomyopathy poses a significant public health burden due to the irreversible loss of functional cardiac tissue. Alternative treatment strategies include creation of three-dimensional (3D) cardiac tissues to both replace and augment injured native tissue. In this study, we utilize a net mold-based method to create a biomaterial-free 3D cardiac tissue and compare it to current methods using biomaterials. Cardiomyocytes, fibroblasts, and endothelial cells were combined using a hanging drop method to create spheroids. For the net mold patch method, spheroids were seeded into a net mold-based system to create biomaterial-free 3D cardiac patches. For the gel patch, spheroids were embedded in a collagen gel. Immunohistochemistry revealed increased alignment, vascularization, collagen I expression, cell viability, and higher density of cells in the net mold patch compared with the gel patch. Furthermore, in vivo testing in a left anterior descending artery ligation rat model found increased ejection fraction and smaller scar area following implantation of the net mold patch. We present a novel and simple reproducible method to create biomaterial-free 3D net mold patches that may potentially improve the treatment of heart failure in the future.
AB - Ischemic cardiomyopathy poses a significant public health burden due to the irreversible loss of functional cardiac tissue. Alternative treatment strategies include creation of three-dimensional (3D) cardiac tissues to both replace and augment injured native tissue. In this study, we utilize a net mold-based method to create a biomaterial-free 3D cardiac tissue and compare it to current methods using biomaterials. Cardiomyocytes, fibroblasts, and endothelial cells were combined using a hanging drop method to create spheroids. For the net mold patch method, spheroids were seeded into a net mold-based system to create biomaterial-free 3D cardiac patches. For the gel patch, spheroids were embedded in a collagen gel. Immunohistochemistry revealed increased alignment, vascularization, collagen I expression, cell viability, and higher density of cells in the net mold patch compared with the gel patch. Furthermore, in vivo testing in a left anterior descending artery ligation rat model found increased ejection fraction and smaller scar area following implantation of the net mold patch. We present a novel and simple reproducible method to create biomaterial-free 3D net mold patches that may potentially improve the treatment of heart failure in the future.
KW - cardiac tissue engineering
KW - hanging drop spheroids
KW - heart failure
KW - mold-based method
UR - http://www.scopus.com/inward/record.url?scp=85064531760&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85064531760&partnerID=8YFLogxK
U2 - 10.1089/ten.tec.2019.0003
DO - 10.1089/ten.tec.2019.0003
M3 - Article
C2 - 30913987
AN - SCOPUS:85064531760
VL - 25
SP - 243
EP - 252
JO - Tissue Engineering - Part C: Methods
JF - Tissue Engineering - Part C: Methods
SN - 1937-3384
IS - 4
ER -