TY - JOUR
T1 - A Net Mold-Based Method of Biomaterial-Free Three-Dimensional Cardiac Tissue Creation
AU - Yang, Bai
AU - Lui, Cecillia
AU - Yeung, Enoch
AU - Matsushita, Hiroshi
AU - Jeyaram, Anjana
AU - Pitaktong, Isaree
AU - Inoue, Takahiro
AU - Mohamed, Zayneb
AU - Ong, Chin Siang
AU - Disilvestre, Deborah
AU - Jay, Steven M.
AU - Tung, Leslie
AU - Tomaselli, Gordon
AU - Ma, Chunye
AU - Hibino, Narutoshi
N1 - Funding Information:
The authors acknowledge funding from the Johns Hopkins Division of Cardiac Surgery Magic that Matters fund.
Publisher Copyright:
© Copyright 2019, Mary Ann Liebert, Inc., publishers 2019.
PY - 2019/4/1
Y1 - 2019/4/1
N2 - Ischemic cardiomyopathy poses a significant public health burden due to the irreversible loss of functional cardiac tissue. Alternative treatment strategies include creation of three-dimensional (3D) cardiac tissues to both replace and augment injured native tissue. In this study, we utilize a net mold-based method to create a biomaterial-free 3D cardiac tissue and compare it to current methods using biomaterials. Cardiomyocytes, fibroblasts, and endothelial cells were combined using a hanging drop method to create spheroids. For the net mold patch method, spheroids were seeded into a net mold-based system to create biomaterial-free 3D cardiac patches. For the gel patch, spheroids were embedded in a collagen gel. Immunohistochemistry revealed increased alignment, vascularization, collagen I expression, cell viability, and higher density of cells in the net mold patch compared with the gel patch. Furthermore, in vivo testing in a left anterior descending artery ligation rat model found increased ejection fraction and smaller scar area following implantation of the net mold patch. We present a novel and simple reproducible method to create biomaterial-free 3D net mold patches that may potentially improve the treatment of heart failure in the future.
AB - Ischemic cardiomyopathy poses a significant public health burden due to the irreversible loss of functional cardiac tissue. Alternative treatment strategies include creation of three-dimensional (3D) cardiac tissues to both replace and augment injured native tissue. In this study, we utilize a net mold-based method to create a biomaterial-free 3D cardiac tissue and compare it to current methods using biomaterials. Cardiomyocytes, fibroblasts, and endothelial cells were combined using a hanging drop method to create spheroids. For the net mold patch method, spheroids were seeded into a net mold-based system to create biomaterial-free 3D cardiac patches. For the gel patch, spheroids were embedded in a collagen gel. Immunohistochemistry revealed increased alignment, vascularization, collagen I expression, cell viability, and higher density of cells in the net mold patch compared with the gel patch. Furthermore, in vivo testing in a left anterior descending artery ligation rat model found increased ejection fraction and smaller scar area following implantation of the net mold patch. We present a novel and simple reproducible method to create biomaterial-free 3D net mold patches that may potentially improve the treatment of heart failure in the future.
KW - cardiac tissue engineering
KW - hanging drop spheroids
KW - heart failure
KW - mold-based method
UR - http://www.scopus.com/inward/record.url?scp=85064531760&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85064531760&partnerID=8YFLogxK
U2 - 10.1089/ten.tec.2019.0003
DO - 10.1089/ten.tec.2019.0003
M3 - Article
C2 - 30913987
AN - SCOPUS:85064531760
VL - 25
SP - 243
EP - 252
JO - Tissue Engineering - Part C: Methods
JF - Tissue Engineering - Part C: Methods
SN - 1937-3384
IS - 4
ER -
