A mouse myeloma variant with a defect in light chain synthesis

S. L. Morrison, Matthew D. Scharff

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A spontaneous assembly variant, B50, has been isolated from the MPC-11 mouse myeloma cell line. This variant synthesizes fewer light chains per cell than the parent resulting in the production of a slight molar excess of heavy chains. These changes are associated with a delay and change in the pathway of assembly and a delay in secretion. Spontaneous revertants of B50 have been obtained, all of which synthesize normal amounts of light chains and assemble and secrete the immunoglobulin molecule through the same pathways and with the same kinetics as the parental cells. A comparison of the tryptic-chymotryptic peptides of the parental, variant and revertant heavy and light chains did not reveal any differences. These studies indicate that variants in mouse myeloma cells can arise with defects in the quantitative expression of the immunoglobulin gene and suggest that the presence of excess light chains facilitates the assembly and secretion of some immunoglobulin molecules.

Original languageEnglish (US)
Pages (from-to)461-465
Number of pages5
JournalEuropean Journal of Immunology
Volume9
Issue number6
StatePublished - 1979

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Light
Immunoglobulins
Immunoglobulin Genes
Cell Line
Peptides

ASJC Scopus subject areas

  • Immunology

Cite this

A mouse myeloma variant with a defect in light chain synthesis. / Morrison, S. L.; Scharff, Matthew D.

In: European Journal of Immunology, Vol. 9, No. 6, 1979, p. 461-465.

Research output: Contribution to journalArticle

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