A modified catalase assay suitable for a plate reader and for the analysis of brain cell cultures

Gerald Cohen, Mimi Kim, Vivian Ogwu

Research output: Contribution to journalArticle

78 Scopus citations

Abstract

The enzyme catalase is present in relatively small amounts in neural tissue. A standard spectrophotometric assay for catalase has been modified to make it suitable both for automated assay with a plate reader and for the analysis of neural cell cultures. Catalase activity is determined by measuring residual hydrogen peroxide after incubation with the enzyme. Ferrous ions and thiocyanate are used for the spectrophotometric determination of hydrogen peroxide. The stable visible absorption of ferrithiocyanate can be measured either with a plate reader or a spectrophotometer. The method assays catalase activity from single wells of a tissue culture plate containing 2-3 mg of embryonic rat mesencephalon.

Original languageEnglish (US)
Pages (from-to)53-56
Number of pages4
JournalJournal of Neuroscience Methods
Volume67
Issue number1
DOIs
StatePublished - Jul 1996
Externally publishedYes

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Keywords

  • Catalase assay
  • Cell culture
  • Mesencephalon
  • Plate reader

ASJC Scopus subject areas

  • Neuroscience(all)

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