A kinesin-related protein, KRP(180), positions prometaphase spindle poles during early sea urchin embryonic cell division

G. C. Rogers, K. K. Chui, E. W. Lee, K. P. Wedaman, D. J. Sharp, G. Holland, R. L. Morris, J. M. Scholey

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

We have investigated the intracellular roles of an Xklp2-related kinesin motor, KRP(180), in positioning spindle poles during early sea urchin embryonic cell division using quantitative, real-time analysis. Immunolocalization reveals that KRP(180) concentrates on microtubules in the central spindle, but is absent from centrosomes. Microinjection of inhibitory antibodies and dominant negative constructs suggest that KRP(180) is not required for the initial separation of spindle poles, but instead functions to transiently position spindle poles specifically during prometaphase.

Original languageEnglish (US)
Pages (from-to)499-511
Number of pages13
JournalJournal of Cell Biology
Volume150
Issue number3
DOIs
StatePublished - Aug 7 2000

Keywords

  • Kinesin
  • Mitosis
  • Sea urchin
  • Xklp2
  • Y-tubulin

ASJC Scopus subject areas

  • Cell Biology

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    Rogers, G. C., Chui, K. K., Lee, E. W., Wedaman, K. P., Sharp, D. J., Holland, G., Morris, R. L., & Scholey, J. M. (2000). A kinesin-related protein, KRP(180), positions prometaphase spindle poles during early sea urchin embryonic cell division. Journal of Cell Biology, 150(3), 499-511. https://doi.org/10.1083/jcb.150.3.499