A kinesin-related protein, KRP(180), positions prometaphase spindle poles during early sea urchin embryonic cell division

G. C. Rogers, K. K. Chui, E. W. Lee, K. P. Wedaman, David J. Sharp, G. Holland, R. L. Morris, J. M. Scholey

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

We have investigated the intracellular roles of an Xklp2-related kinesin motor, KRP(180), in positioning spindle poles during early sea urchin embryonic cell division using quantitative, real-time analysis. Immunolocalization reveals that KRP(180) concentrates on microtubules in the central spindle, but is absent from centrosomes. Microinjection of inhibitory antibodies and dominant negative constructs suggest that KRP(180) is not required for the initial separation of spindle poles, but instead functions to transiently position spindle poles specifically during prometaphase.

Original languageEnglish (US)
Pages (from-to)499-511
Number of pages13
JournalJournal of Cell Biology
Volume150
Issue number3
DOIs
StatePublished - Aug 7 2000
Externally publishedYes

Fingerprint

Prometaphase
Spindle Poles
Kinesin
Sea Urchins
Cell Division
Centrosome
Proteins
Microinjections
Microtubules
Antibodies

Keywords

  • Kinesin
  • Mitosis
  • Sea urchin
  • Xklp2
  • Y-tubulin

ASJC Scopus subject areas

  • Cell Biology

Cite this

A kinesin-related protein, KRP(180), positions prometaphase spindle poles during early sea urchin embryonic cell division. / Rogers, G. C.; Chui, K. K.; Lee, E. W.; Wedaman, K. P.; Sharp, David J.; Holland, G.; Morris, R. L.; Scholey, J. M.

In: Journal of Cell Biology, Vol. 150, No. 3, 07.08.2000, p. 499-511.

Research output: Contribution to journalArticle

Rogers, G. C. ; Chui, K. K. ; Lee, E. W. ; Wedaman, K. P. ; Sharp, David J. ; Holland, G. ; Morris, R. L. ; Scholey, J. M. / A kinesin-related protein, KRP(180), positions prometaphase spindle poles during early sea urchin embryonic cell division. In: Journal of Cell Biology. 2000 ; Vol. 150, No. 3. pp. 499-511.
@article{255c93306e7e4329ba76e222d81c9fe6,
title = "A kinesin-related protein, KRP(180), positions prometaphase spindle poles during early sea urchin embryonic cell division",
abstract = "We have investigated the intracellular roles of an Xklp2-related kinesin motor, KRP(180), in positioning spindle poles during early sea urchin embryonic cell division using quantitative, real-time analysis. Immunolocalization reveals that KRP(180) concentrates on microtubules in the central spindle, but is absent from centrosomes. Microinjection of inhibitory antibodies and dominant negative constructs suggest that KRP(180) is not required for the initial separation of spindle poles, but instead functions to transiently position spindle poles specifically during prometaphase.",
keywords = "Kinesin, Mitosis, Sea urchin, Xklp2, Y-tubulin",
author = "Rogers, {G. C.} and Chui, {K. K.} and Lee, {E. W.} and Wedaman, {K. P.} and Sharp, {David J.} and G. Holland and Morris, {R. L.} and Scholey, {J. M.}",
year = "2000",
month = "8",
day = "7",
doi = "10.1083/jcb.150.3.499",
language = "English (US)",
volume = "150",
pages = "499--511",
journal = "Journal of Cell Biology",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "3",

}

TY - JOUR

T1 - A kinesin-related protein, KRP(180), positions prometaphase spindle poles during early sea urchin embryonic cell division

AU - Rogers, G. C.

AU - Chui, K. K.

AU - Lee, E. W.

AU - Wedaman, K. P.

AU - Sharp, David J.

AU - Holland, G.

AU - Morris, R. L.

AU - Scholey, J. M.

PY - 2000/8/7

Y1 - 2000/8/7

N2 - We have investigated the intracellular roles of an Xklp2-related kinesin motor, KRP(180), in positioning spindle poles during early sea urchin embryonic cell division using quantitative, real-time analysis. Immunolocalization reveals that KRP(180) concentrates on microtubules in the central spindle, but is absent from centrosomes. Microinjection of inhibitory antibodies and dominant negative constructs suggest that KRP(180) is not required for the initial separation of spindle poles, but instead functions to transiently position spindle poles specifically during prometaphase.

AB - We have investigated the intracellular roles of an Xklp2-related kinesin motor, KRP(180), in positioning spindle poles during early sea urchin embryonic cell division using quantitative, real-time analysis. Immunolocalization reveals that KRP(180) concentrates on microtubules in the central spindle, but is absent from centrosomes. Microinjection of inhibitory antibodies and dominant negative constructs suggest that KRP(180) is not required for the initial separation of spindle poles, but instead functions to transiently position spindle poles specifically during prometaphase.

KW - Kinesin

KW - Mitosis

KW - Sea urchin

KW - Xklp2

KW - Y-tubulin

UR - http://www.scopus.com/inward/record.url?scp=0034618133&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034618133&partnerID=8YFLogxK

U2 - 10.1083/jcb.150.3.499

DO - 10.1083/jcb.150.3.499

M3 - Article

VL - 150

SP - 499

EP - 511

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 3

ER -