A human autoimmune protein associated with U1 RNA contains a region of homology that is cross-reactive with retroviral p30gag antigen

Charles C. Query, Jack D. Keene

Research output: Contribution to journalArticle

237 Citations (Scopus)

Abstract

cDNA encoding a 70 kd protein (70K) associated with U1 small nuclear ribonucleoprotein (snRNP) was cloned from a human brain-stem library using autoantibodies from patients with connective tissue disease. The cDNA-derived amino acid sequence contains 23 residues homologous to a region of murine leukemia virus group-specific antigen p30gag. The homology resides in an antigenic portion of the 70K protein and is defined by a core consensus sequence, ETPEEREERRR, that occurs as a tandem repeat in p30gag of most mammalian type C retroviruses. Anti-p30gag antibodies recognized a recombinant 70K-LacZ fusion protein as well as U1 snRNPs. Using synthetic peptides as competitors, we demonstrated that the region of homology encompasses the site of immunological cross-reactivity. Thus autoantibodies against U1 snRNPs were elicited by immunization with p30gag. On the basis of these findings, we suggest a role for retroviruses in the initiation of autoimmunity.

Original languageEnglish (US)
Pages (from-to)211-220
Number of pages10
JournalCell
Volume51
Issue number2
DOIs
StatePublished - Oct 23 1987
Externally publishedYes

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Antigens
Proteins
Autoantibodies
Complementary DNA
U1 Small Nuclear Ribonucleoproteins
Gammaretrovirus
Immunization
Murine Leukemia Viruses
Tandem Repeat Sequences
Connective Tissue Diseases
Consensus Sequence
Retroviridae
Autoimmunity
Viruses
Brain Stem
Anti-Idiotypic Antibodies
Amino Acid Sequence
Brain
Fusion reactions
U1 small nuclear RNA

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

A human autoimmune protein associated with U1 RNA contains a region of homology that is cross-reactive with retroviral p30gag antigen. / Query, Charles C.; Keene, Jack D.

In: Cell, Vol. 51, No. 2, 23.10.1987, p. 211-220.

Research output: Contribution to journalArticle

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