A Forward Genetic Screen in Zebrafish Identifies the G-Protein-Coupled Receptor CaSR as a Modulator of Sensorimotor Decision Making

Roshan A. Jain, Marc A. Wolman, Kurt C. Marsden, Jessica C. Nelson, Hannah Shoenhard, Fabio A. Echeverry, Christina Szi, Hannah Bell, Julianne Skinner, Emilia N. Cobbs, Keisuke Sawada, Amy D. Zamora, Alberto E. Pereda, Michael Granato

Research output: Contribution to journalArticle

Abstract

Animals continuously integrate sensory information and select contextually appropriate responses. Here, we show that zebrafish larvae select a behavioral response to acoustic stimuli from a pre-existing choice repertoire in a context-dependent manner. We demonstrate that this sensorimotor choice is modulated by stimulus quality and history, as well as by neuromodulatory systems—all hallmarks of more complex decision making. Moreover, from a genetic screen coupled with whole-genome sequencing, we identified eight mutants with deficits in this sensorimotor choice, including mutants of the vertebrate-specific G-protein-coupled extracellular calcium-sensing receptor (CaSR), whose function in the nervous system is not well understood. We demonstrate that CaSR promotes sensorimotor decision making acutely through Gαi/o and Gαq/11 signaling, modulated by clathrin-mediated endocytosis. Combined, our results identify the first set of genes critical for behavioral choice modulation in a vertebrate and reveal an unexpected critical role for CaSR in sensorimotor decision making. Using larval zebrafish, Jain et al. establish and validate a simple sensorimotor decision-making paradigm. Using this paradigm, they perform a forward genetic screen that identifies the first set of vertebrate sensorimotor decision-making genes, including the calcium-sensing receptor, which acts as a bidirectional regulator of sensorimotor choice.

Original languageEnglish (US)
JournalCurrent Biology
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

Calcium-Sensing Receptors
Zebrafish
G-Protein-Coupled Receptors
Danio rerio
Modulators
decision making
Decision Making
Decision making
calcium
receptors
Vertebrates
Genes
vertebrates
clathrin
mutants
Clathrin
Neurology
endocytosis
Endocytosis
G-proteins

Keywords

  • AP2S1
  • behavior choice
  • calcium-sensing receptor
  • CaSR
  • decision making
  • genetic screen
  • startle response
  • zebrafish

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Jain, R. A., Wolman, M. A., Marsden, K. C., Nelson, J. C., Shoenhard, H., Echeverry, F. A., ... Granato, M. (Accepted/In press). A Forward Genetic Screen in Zebrafish Identifies the G-Protein-Coupled Receptor CaSR as a Modulator of Sensorimotor Decision Making. Current Biology. https://doi.org/10.1016/j.cub.2018.03.025

A Forward Genetic Screen in Zebrafish Identifies the G-Protein-Coupled Receptor CaSR as a Modulator of Sensorimotor Decision Making. / Jain, Roshan A.; Wolman, Marc A.; Marsden, Kurt C.; Nelson, Jessica C.; Shoenhard, Hannah; Echeverry, Fabio A.; Szi, Christina; Bell, Hannah; Skinner, Julianne; Cobbs, Emilia N.; Sawada, Keisuke; Zamora, Amy D.; Pereda, Alberto E.; Granato, Michael.

In: Current Biology, 01.01.2018.

Research output: Contribution to journalArticle

Jain, RA, Wolman, MA, Marsden, KC, Nelson, JC, Shoenhard, H, Echeverry, FA, Szi, C, Bell, H, Skinner, J, Cobbs, EN, Sawada, K, Zamora, AD, Pereda, AE & Granato, M 2018, 'A Forward Genetic Screen in Zebrafish Identifies the G-Protein-Coupled Receptor CaSR as a Modulator of Sensorimotor Decision Making', Current Biology. https://doi.org/10.1016/j.cub.2018.03.025
Jain, Roshan A. ; Wolman, Marc A. ; Marsden, Kurt C. ; Nelson, Jessica C. ; Shoenhard, Hannah ; Echeverry, Fabio A. ; Szi, Christina ; Bell, Hannah ; Skinner, Julianne ; Cobbs, Emilia N. ; Sawada, Keisuke ; Zamora, Amy D. ; Pereda, Alberto E. ; Granato, Michael. / A Forward Genetic Screen in Zebrafish Identifies the G-Protein-Coupled Receptor CaSR as a Modulator of Sensorimotor Decision Making. In: Current Biology. 2018.
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