A Fluorescently Labeled Marburg Virus Glycoprotein as a New Tool to Study Viral Transport and Assembly

Eva-Maria Mittler, Gordian Schudt, Sandro Halwe, Cornelius Rohde, Stephan Becker

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The single surface glycoprotein (GP) of filoviruses is indispensable for recognition of its cellular receptor and infection of target cells. To study the intracellular trafficking of GP by using live-cell imaging, the mucin-like domain of Marburg virus (MARV) GP was replaced by the fluorophore mCherry (GP∆MLD_mCherry). Intracellular distribution, surface transport, and recruitment of GP∆MLD_mCherry into virus-like particles were similar to observations for wild-type GP. Using reverse genetics, we generated a recombinant MARV expressing GP∆MLD_mCherry (recMARV MARVGP∆MLD_mCherry). Time-lapse microscopy of recMARV MARVGP∆MLD_mCherry-infected cells revealed that GP∆MLD_mCherry-positive vesicles were transported to the cell surface in a tubulin-dependent manner. Moreover, dual-color live-cell imaging revealed cotransport of GPΔMLD_mCherry and VP40 and their colocalization at the plasma membrane. In this proof-of-concept study we showed that the newly developed GP∆MLD_mCherry is a promising tool to elucidate intracellular trafficking and assembly pathways of MARV.

Original languageEnglish (US)
Pages (from-to)S318-S326
JournalThe Journal of infectious diseases
Volume218
Issue number5
DOIs
StatePublished - Nov 22 2018
Externally publishedYes

Fingerprint

Marburgvirus
Virus Assembly
Glycoproteins
Reverse Genetics
Membrane Glycoproteins
Mucins
Tubulin
Virion
Microscopy
Color
Cell Membrane
Infection

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

Cite this

A Fluorescently Labeled Marburg Virus Glycoprotein as a New Tool to Study Viral Transport and Assembly. / Mittler, Eva-Maria; Schudt, Gordian; Halwe, Sandro; Rohde, Cornelius; Becker, Stephan.

In: The Journal of infectious diseases, Vol. 218, No. 5, 22.11.2018, p. S318-S326.

Research output: Contribution to journalArticle

Mittler, Eva-Maria ; Schudt, Gordian ; Halwe, Sandro ; Rohde, Cornelius ; Becker, Stephan. / A Fluorescently Labeled Marburg Virus Glycoprotein as a New Tool to Study Viral Transport and Assembly. In: The Journal of infectious diseases. 2018 ; Vol. 218, No. 5. pp. S318-S326.
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