A comparative hybridization analysis of yeast DNA with Paramecium parafusin- and different phosphoglucomutase-specific probes

E. Wyroba, B. H. Satir

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Molecular probes designed for the parafusin (PFUS), the Paramecium exocytic-sensitive phosphoglyco-protein, gave distinct hybridization patterns in Saccharomyces cerevisiae genomic DNA when compared with different phosphoglucomutase specific probes. These include two probes identical to segments of yeast phosphoglucomutase (PGM) genes 1 and 2. Neither of the PGM probes revealed the 7.4 and 5.9 kb fragments in Bgl II-cut yeast DNA digest detected with the 1.6 kb cloned PFUS cDNA and oligonucleotide constructed to the PFUS region (insertion 3 -1-3) not found in other species. PCR amplification with PFUS-specific primers generated yeast DNA-species of the predicted molecular size which hybridized to the I-3 probe. A search of the yeast genome database produced an unassigned nucleotide sequence that showed 55% identity to parafusin gene and 37% identity to PGM2 (the major isoform of yeast phosphoglucomutase) within the amplified region.

Original languageEnglish (US)
Pages (from-to)683-690
Number of pages8
JournalBiochemistry and Cell Biology
Volume78
Issue number6
DOIs
Publication statusPublished - Dec 1 2000

    Fingerprint

Keywords

  • Hybridization
  • PCR
  • Parafusin
  • Phosphoglucomutase
  • Yeast

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this