A candidate metastasis-associated DNA marker for ductal mammary carcinoma.

Patnala Mohan R Achary, Hui Zhao, Zuoheng Fan, Swarna Gogineni, Venkat R. Pulijaal, Lawrence H. Herbst, Panna S. Mahadevia, Joan G. Jones, Harold P. Klinger, Bhadrasain Vikram

Research output: Contribution to journalArticle

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Abstract

BACKGROUND: Molecular genetic markers to identify the 13% lymph node-negative mammary carcinomas that are prone to develop metastases would clearly be of considerable value in indicating those cases in need of early aggressive therapy. METHODS: Representational difference analysis was used in an attempt to identify genetic alterations related to breast cancer metastasis by comparing genomic DNA from microdissected normal cells and from metastatic cells of ductal breast carcinoma patients. RESULTS: Representational difference analysis products yielded 10 unique metastasis-associated DNA sequences (MADS), i.e. products apparently lost in metastatic cell DNA. Of these sequences, MADS-IX was found to be lost in the transition from primary to metastasis in two out of five ductal breast carcinoma cases. This sequence was localized on chromosome 10q21 by radiation hybrid mapping and fluorescence in situ hybridization. The PTEN gene, which is also located on chromosome 10q, was detected to be present by PCR in all five cases. On the contrary, a breast carcinoma cell line, HCC-1937, which has homozygous loss of a region encompassing the PTEN gene, showed the presence of MADS-IX. PCR screening of three additional breast carcinoma cell lines with known losses in specific chromosomal regions also showed the presence of MADS-IX. CONCLUSION: These data suggest that MADS-IX possibly is part of a novel candidate metastasis-associated gene located close to the PTEN gene on chromosome 10q. The first set of PCR screening in five patient samples indicates that it could be used as a molecular marker for ductal mammary metastasis.

Original languageEnglish (US)
JournalBreast cancer research : BCR
Volume5
Issue number2
StatePublished - 2003

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Carcinoma, Ductal, Breast
Genetic Markers
Neoplasm Metastasis
Breast Neoplasms
Chromosomes
Polymerase Chain Reaction
Genes
Radiation Hybrid Mapping
Cell Line
DNA
Secondary Prevention
Fluorescence In Situ Hybridization
Molecular Biology
Breast
Lymph Nodes

Cite this

Achary, P. M. R., Zhao, H., Fan, Z., Gogineni, S., Pulijaal, V. R., Herbst, L. H., ... Vikram, B. (2003). A candidate metastasis-associated DNA marker for ductal mammary carcinoma. Breast cancer research : BCR, 5(2).

A candidate metastasis-associated DNA marker for ductal mammary carcinoma. / Achary, Patnala Mohan R; Zhao, Hui; Fan, Zuoheng; Gogineni, Swarna; Pulijaal, Venkat R.; Herbst, Lawrence H.; Mahadevia, Panna S.; Jones, Joan G.; Klinger, Harold P.; Vikram, Bhadrasain.

In: Breast cancer research : BCR, Vol. 5, No. 2, 2003.

Research output: Contribution to journalArticle

Achary, PMR, Zhao, H, Fan, Z, Gogineni, S, Pulijaal, VR, Herbst, LH, Mahadevia, PS, Jones, JG, Klinger, HP & Vikram, B 2003, 'A candidate metastasis-associated DNA marker for ductal mammary carcinoma.', Breast cancer research : BCR, vol. 5, no. 2.
Achary, Patnala Mohan R ; Zhao, Hui ; Fan, Zuoheng ; Gogineni, Swarna ; Pulijaal, Venkat R. ; Herbst, Lawrence H. ; Mahadevia, Panna S. ; Jones, Joan G. ; Klinger, Harold P. ; Vikram, Bhadrasain. / A candidate metastasis-associated DNA marker for ductal mammary carcinoma. In: Breast cancer research : BCR. 2003 ; Vol. 5, No. 2.
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abstract = "BACKGROUND: Molecular genetic markers to identify the 13{\%} lymph node-negative mammary carcinomas that are prone to develop metastases would clearly be of considerable value in indicating those cases in need of early aggressive therapy. METHODS: Representational difference analysis was used in an attempt to identify genetic alterations related to breast cancer metastasis by comparing genomic DNA from microdissected normal cells and from metastatic cells of ductal breast carcinoma patients. RESULTS: Representational difference analysis products yielded 10 unique metastasis-associated DNA sequences (MADS), i.e. products apparently lost in metastatic cell DNA. Of these sequences, MADS-IX was found to be lost in the transition from primary to metastasis in two out of five ductal breast carcinoma cases. This sequence was localized on chromosome 10q21 by radiation hybrid mapping and fluorescence in situ hybridization. The PTEN gene, which is also located on chromosome 10q, was detected to be present by PCR in all five cases. On the contrary, a breast carcinoma cell line, HCC-1937, which has homozygous loss of a region encompassing the PTEN gene, showed the presence of MADS-IX. PCR screening of three additional breast carcinoma cell lines with known losses in specific chromosomal regions also showed the presence of MADS-IX. CONCLUSION: These data suggest that MADS-IX possibly is part of a novel candidate metastasis-associated gene located close to the PTEN gene on chromosome 10q. The first set of PCR screening in five patient samples indicates that it could be used as a molecular marker for ductal mammary metastasis.",
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AU - Zhao, Hui

AU - Fan, Zuoheng

AU - Gogineni, Swarna

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AU - Herbst, Lawrence H.

AU - Mahadevia, Panna S.

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AU - Klinger, Harold P.

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N2 - BACKGROUND: Molecular genetic markers to identify the 13% lymph node-negative mammary carcinomas that are prone to develop metastases would clearly be of considerable value in indicating those cases in need of early aggressive therapy. METHODS: Representational difference analysis was used in an attempt to identify genetic alterations related to breast cancer metastasis by comparing genomic DNA from microdissected normal cells and from metastatic cells of ductal breast carcinoma patients. RESULTS: Representational difference analysis products yielded 10 unique metastasis-associated DNA sequences (MADS), i.e. products apparently lost in metastatic cell DNA. Of these sequences, MADS-IX was found to be lost in the transition from primary to metastasis in two out of five ductal breast carcinoma cases. This sequence was localized on chromosome 10q21 by radiation hybrid mapping and fluorescence in situ hybridization. The PTEN gene, which is also located on chromosome 10q, was detected to be present by PCR in all five cases. On the contrary, a breast carcinoma cell line, HCC-1937, which has homozygous loss of a region encompassing the PTEN gene, showed the presence of MADS-IX. PCR screening of three additional breast carcinoma cell lines with known losses in specific chromosomal regions also showed the presence of MADS-IX. CONCLUSION: These data suggest that MADS-IX possibly is part of a novel candidate metastasis-associated gene located close to the PTEN gene on chromosome 10q. The first set of PCR screening in five patient samples indicates that it could be used as a molecular marker for ductal mammary metastasis.

AB - BACKGROUND: Molecular genetic markers to identify the 13% lymph node-negative mammary carcinomas that are prone to develop metastases would clearly be of considerable value in indicating those cases in need of early aggressive therapy. METHODS: Representational difference analysis was used in an attempt to identify genetic alterations related to breast cancer metastasis by comparing genomic DNA from microdissected normal cells and from metastatic cells of ductal breast carcinoma patients. RESULTS: Representational difference analysis products yielded 10 unique metastasis-associated DNA sequences (MADS), i.e. products apparently lost in metastatic cell DNA. Of these sequences, MADS-IX was found to be lost in the transition from primary to metastasis in two out of five ductal breast carcinoma cases. This sequence was localized on chromosome 10q21 by radiation hybrid mapping and fluorescence in situ hybridization. The PTEN gene, which is also located on chromosome 10q, was detected to be present by PCR in all five cases. On the contrary, a breast carcinoma cell line, HCC-1937, which has homozygous loss of a region encompassing the PTEN gene, showed the presence of MADS-IX. PCR screening of three additional breast carcinoma cell lines with known losses in specific chromosomal regions also showed the presence of MADS-IX. CONCLUSION: These data suggest that MADS-IX possibly is part of a novel candidate metastasis-associated gene located close to the PTEN gene on chromosome 10q. The first set of PCR screening in five patient samples indicates that it could be used as a molecular marker for ductal mammary metastasis.

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