A Basis for Fluoropyrimidine-Induced Antagonism to Methotrexate in Ehrlich Ascites Tumor Cells in Vitro

Donnell Bowen, J. Courtl, I. David Goldman

Research output: Contribution to journalArticle

29 Scopus citations


The inhibitory effect of methotrexate on [3 H]deoxyuridine incorporation into DNA is reduced as the basal rate of this reaction is inhibited by pretreatment of Ehrlich ascites tumor cells with fluoropyrimidines. This observation is a basis for fluoropyrimidine-methotrexate antagonism in anticancer regimens and supports the concept that the sensitivity of thymidylate synthesis in tumor cells to methotrexate is related, in part, to the basal rate of thymidylate synthesis from deoxyuridylate.

Original languageEnglish (US)
Pages (from-to)219-222
Number of pages4
JournalCancer Research
Issue number1
Publication statusPublished - Jan 1978
Externally publishedYes


ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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