5-HT3 receptor ion size selectivity is a property of the transmembrane channel, not the cytoplasmic vestibule portals

Nicole K. McKinnon, David C. Reeves, Myles Akabas

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

5-HT3A receptors select among permeant ions based on size and charge. The membrane-associated (MA) helix lines the portals into the channel's cytoplasmic vestibule in the 4-Å resolution structure of the homologous acetylcholine receptor. 5-HT3A MA helix residues are important determinants of single-channel conductance. It is unknown whether the portals into the cytoplasmic vestibule also determine the size selectivity of permeant ions. We sought to determine whether the portals form the size selectivity filter. Recently, we showed that channels functioned when the entire 5-HT3A M3-M4 loop was replaced by the heptapeptide M3-M4 loop sequence from GLIC, a bacterial Cys-loop neurotransmitter gated ion channel homologue from Gloebacter violaceus. We used homomeric 5-HT3A receptors with either a wild-type (WT) M3-M4 loop or the chimeric heptapeptide (5-HT3A-glvM3M4) loop, i.e., with or without portals. In Na +-containing buffer, the WT receptor current-voltage relationship was inwardly rectifying. In contrast, the 5-HT3A-glvM3M4 construct had a negative slope conductance region at voltages less than -80 mV. Glutamine substitution for the heptapeptide M3-M4 loop arginine eliminated the negative slope conductance region. We measured the relative permeabilities and conductances of a series of inorganic and organic cations ranging from 0.9 to 4.5 Å in radius (Li +, Na +, ammonium, methylammonium, ethanolammonium, 2-methylethanolammonium, dimethylammonium, diethanolammonium, tetramethylammonium, choline, tris [hydroxymethyl] aminomethane, and N-methyl-d-glucamine). Both constructs had measurable conductances with Li +, ammonium, and methylammonium (size range of 0.9-1.8-Å radius). Many of the organic cations >2.4 Å acted as competitive antagonists complicating measurement of conductance ratios. Analysis of the permeability ratios by excluded volume theory indicates that the minimal pore radius for 5-HT3A and 5-HT3-glvM3M4 receptors was similar, ~5 Å. We infer that the 5-HT3A size selectivity filter is located in the transmembrane channel and not in the portals into the cytoplasmic vestibule. Thus, the determinants of size selectivity and conductance are located in physically distinct regions of the channel protein.

Original languageEnglish (US)
Pages (from-to)453-466
Number of pages14
JournalJournal of General Physiology
Volume138
Issue number4
DOIs
StatePublished - Oct 2011

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Receptors, Serotonin, 5-HT3
Ammonium Compounds
Cations
Permeability
Ions
Tromethamine
Membranes
Cholinergic Receptors
Choline
Glutamine
Ion Channels
Neurotransmitter Agents
Arginine
Buffers
Proteins
methylamine
tetramethylammonium

ASJC Scopus subject areas

  • Physiology

Cite this

5-HT3 receptor ion size selectivity is a property of the transmembrane channel, not the cytoplasmic vestibule portals. / McKinnon, Nicole K.; Reeves, David C.; Akabas, Myles.

In: Journal of General Physiology, Vol. 138, No. 4, 10.2011, p. 453-466.

Research output: Contribution to journalArticle

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abstract = "5-HT3A receptors select among permeant ions based on size and charge. The membrane-associated (MA) helix lines the portals into the channel's cytoplasmic vestibule in the 4-{\AA} resolution structure of the homologous acetylcholine receptor. 5-HT3A MA helix residues are important determinants of single-channel conductance. It is unknown whether the portals into the cytoplasmic vestibule also determine the size selectivity of permeant ions. We sought to determine whether the portals form the size selectivity filter. Recently, we showed that channels functioned when the entire 5-HT3A M3-M4 loop was replaced by the heptapeptide M3-M4 loop sequence from GLIC, a bacterial Cys-loop neurotransmitter gated ion channel homologue from Gloebacter violaceus. We used homomeric 5-HT3A receptors with either a wild-type (WT) M3-M4 loop or the chimeric heptapeptide (5-HT3A-glvM3M4) loop, i.e., with or without portals. In Na +-containing buffer, the WT receptor current-voltage relationship was inwardly rectifying. In contrast, the 5-HT3A-glvM3M4 construct had a negative slope conductance region at voltages less than -80 mV. Glutamine substitution for the heptapeptide M3-M4 loop arginine eliminated the negative slope conductance region. We measured the relative permeabilities and conductances of a series of inorganic and organic cations ranging from 0.9 to 4.5 {\AA} in radius (Li +, Na +, ammonium, methylammonium, ethanolammonium, 2-methylethanolammonium, dimethylammonium, diethanolammonium, tetramethylammonium, choline, tris [hydroxymethyl] aminomethane, and N-methyl-d-glucamine). Both constructs had measurable conductances with Li +, ammonium, and methylammonium (size range of 0.9-1.8-{\AA} radius). Many of the organic cations >2.4 {\AA} acted as competitive antagonists complicating measurement of conductance ratios. Analysis of the permeability ratios by excluded volume theory indicates that the minimal pore radius for 5-HT3A and 5-HT3-glvM3M4 receptors was similar, ~5 {\AA}. We infer that the 5-HT3A size selectivity filter is located in the transmembrane channel and not in the portals into the cytoplasmic vestibule. Thus, the determinants of size selectivity and conductance are located in physically distinct regions of the channel protein.",
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N2 - 5-HT3A receptors select among permeant ions based on size and charge. The membrane-associated (MA) helix lines the portals into the channel's cytoplasmic vestibule in the 4-Å resolution structure of the homologous acetylcholine receptor. 5-HT3A MA helix residues are important determinants of single-channel conductance. It is unknown whether the portals into the cytoplasmic vestibule also determine the size selectivity of permeant ions. We sought to determine whether the portals form the size selectivity filter. Recently, we showed that channels functioned when the entire 5-HT3A M3-M4 loop was replaced by the heptapeptide M3-M4 loop sequence from GLIC, a bacterial Cys-loop neurotransmitter gated ion channel homologue from Gloebacter violaceus. We used homomeric 5-HT3A receptors with either a wild-type (WT) M3-M4 loop or the chimeric heptapeptide (5-HT3A-glvM3M4) loop, i.e., with or without portals. In Na +-containing buffer, the WT receptor current-voltage relationship was inwardly rectifying. In contrast, the 5-HT3A-glvM3M4 construct had a negative slope conductance region at voltages less than -80 mV. Glutamine substitution for the heptapeptide M3-M4 loop arginine eliminated the negative slope conductance region. We measured the relative permeabilities and conductances of a series of inorganic and organic cations ranging from 0.9 to 4.5 Å in radius (Li +, Na +, ammonium, methylammonium, ethanolammonium, 2-methylethanolammonium, dimethylammonium, diethanolammonium, tetramethylammonium, choline, tris [hydroxymethyl] aminomethane, and N-methyl-d-glucamine). Both constructs had measurable conductances with Li +, ammonium, and methylammonium (size range of 0.9-1.8-Å radius). Many of the organic cations >2.4 Å acted as competitive antagonists complicating measurement of conductance ratios. Analysis of the permeability ratios by excluded volume theory indicates that the minimal pore radius for 5-HT3A and 5-HT3-glvM3M4 receptors was similar, ~5 Å. We infer that the 5-HT3A size selectivity filter is located in the transmembrane channel and not in the portals into the cytoplasmic vestibule. Thus, the determinants of size selectivity and conductance are located in physically distinct regions of the channel protein.

AB - 5-HT3A receptors select among permeant ions based on size and charge. The membrane-associated (MA) helix lines the portals into the channel's cytoplasmic vestibule in the 4-Å resolution structure of the homologous acetylcholine receptor. 5-HT3A MA helix residues are important determinants of single-channel conductance. It is unknown whether the portals into the cytoplasmic vestibule also determine the size selectivity of permeant ions. We sought to determine whether the portals form the size selectivity filter. Recently, we showed that channels functioned when the entire 5-HT3A M3-M4 loop was replaced by the heptapeptide M3-M4 loop sequence from GLIC, a bacterial Cys-loop neurotransmitter gated ion channel homologue from Gloebacter violaceus. We used homomeric 5-HT3A receptors with either a wild-type (WT) M3-M4 loop or the chimeric heptapeptide (5-HT3A-glvM3M4) loop, i.e., with or without portals. In Na +-containing buffer, the WT receptor current-voltage relationship was inwardly rectifying. In contrast, the 5-HT3A-glvM3M4 construct had a negative slope conductance region at voltages less than -80 mV. Glutamine substitution for the heptapeptide M3-M4 loop arginine eliminated the negative slope conductance region. We measured the relative permeabilities and conductances of a series of inorganic and organic cations ranging from 0.9 to 4.5 Å in radius (Li +, Na +, ammonium, methylammonium, ethanolammonium, 2-methylethanolammonium, dimethylammonium, diethanolammonium, tetramethylammonium, choline, tris [hydroxymethyl] aminomethane, and N-methyl-d-glucamine). Both constructs had measurable conductances with Li +, ammonium, and methylammonium (size range of 0.9-1.8-Å radius). Many of the organic cations >2.4 Å acted as competitive antagonists complicating measurement of conductance ratios. Analysis of the permeability ratios by excluded volume theory indicates that the minimal pore radius for 5-HT3A and 5-HT3-glvM3M4 receptors was similar, ~5 Å. We infer that the 5-HT3A size selectivity filter is located in the transmembrane channel and not in the portals into the cytoplasmic vestibule. Thus, the determinants of size selectivity and conductance are located in physically distinct regions of the channel protein.

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