β-adrenergic stimulation causes cardiocyte apoptosis

Influence of tachycardia and hypertrophy

Yukitaka Shizukuda, Peter M. Buttrick, David L. Geenen, Alain C. Borczuk, Richard N. Kitsis, Edmund H. Sonnenblick

Research output: Contribution to journalArticle

144 Citations (Scopus)

Abstract

To establish whether catecholamines per se in the absence of significant increases in systolic load induce myocardial damage via apoptosis, rats were treated with vehicle or isoproterenol (400 μg·kg-1·h-1). Apoptotic cardiocytes (Apo) were identified in paraffin-embedded sections using terminal deoxynucleotide transferase-mediated dUTP nick end labeling. Results were confirmed using an independent ligase assay. Systolic blood pressures were comparable in isoproterenol-treated and control rats. Twenty-four hours of treatment with isoproterenol resulted in significant numbers of Apo compared with control [7.9 ± 2.5 vs. 0.3 ± 0.3 (SE) cm-2, P < 0.05]. A cohort of animals was subjected to ventricular pacing to induce a tachycardia equivalent to that induced by isoproterenol, and these animals did not show an increase in Apo. The left ventricular hypertrophy induced by 2 wk of abdominal aortic banding also increased Apo (∼7.2-fold); however, 24 h of isoproterenol infusion did not induce additional Apo in these rats. Thus catecholamines, in the absence of altered systolic load, induce Apo which is not mediated solely by tachycardia. Left ventricular hypertrophy secondary to abdominal aortic banding is associated with Apo, but this does not increase sensitivity to isoproterenol-induced Apo.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume44
Issue number3
StatePublished - Sep 1998

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Isoproterenol
Tachycardia
Adrenergic Agents
Hypertrophy
Apoptosis
Left Ventricular Hypertrophy
Catecholamines
Blood Pressure
Ligases
Transferases
Paraffin

Keywords

  • Cardiac pacing
  • Catecholamines
  • Ligase reaction
  • Myocardial cell death
  • Rats

ASJC Scopus subject areas

  • Physiology

Cite this

β-adrenergic stimulation causes cardiocyte apoptosis : Influence of tachycardia and hypertrophy. / Shizukuda, Yukitaka; Buttrick, Peter M.; Geenen, David L.; Borczuk, Alain C.; Kitsis, Richard N.; Sonnenblick, Edmund H.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 44, No. 3, 09.1998.

Research output: Contribution to journalArticle

Shizukuda, Yukitaka ; Buttrick, Peter M. ; Geenen, David L. ; Borczuk, Alain C. ; Kitsis, Richard N. ; Sonnenblick, Edmund H. / β-adrenergic stimulation causes cardiocyte apoptosis : Influence of tachycardia and hypertrophy. In: American Journal of Physiology - Heart and Circulatory Physiology. 1998 ; Vol. 44, No. 3.
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