αb-crystallin/sHSP protects cytochrome c and mitochondrial function against oxidative stress in lens and retinal cells

Rebecca S. Estrada, Wanda Lee Kantorow, Daniel C. Chauss, Jianning Wei, Lisa A. Brennan, Marc Kantorow

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Background: αB-crystallin/sHSP protects cells against oxidative stress damage. Here, we mechanistically examined its ability to preserve mitochondrial function in lens and retinal cells and protect cytochrome c under oxidative stress conditions. Methods: αB-crystallin/sHSP was localized in human lens (HLE-B3) and retinal (ARPE-19) cells. αB-crystallin/sHSP was stably over-expressed and its ability to preserve mitochondrial membrane potential under oxidative stress conditions was monitored. Interactions between αB-crystallin/sHSP and cytochrome c were examined by fluorescent resonance energy transfer (FRET) and by co-immune precipitation. The ability of αB-crystallin/sHSP to protect cytochrome c against methionine-80 oxidation was monitored. Results: αB-crystallin/sHSP is present in the mitochondria of lens and retinal cells and is translocated to the mitochondria under oxidative conditions. αB-crystallin/sHSP specifically interacts with cytochrome c in vitro and in vivo and its overexpression preserves mitochondrial membrane potential under oxidative stress conditions. αB-crystallin/sHSP directly protects cytochrome c against oxidation. General significance: These data demonstrate that αB-crystallin/sHSP maintains lens and retinal cells under oxidative stress conditions at least in part by preserving mitochondrial function and by protecting cytochrome c against oxidation. Since oxidative stress and loss of mitochondrial function are associated with eye lens cataract and age-related macular degeneration, loss of these αB-crystallin/sHSP functions likely plays a key role in the development of these diseases. αB-crystallin/sHSP is expressed throughout the body and its ability to maintain mitochondrial function is likely important for the prevention of multiple degenerative diseases.

Original languageEnglish (US)
Pages (from-to)921-930
Number of pages10
JournalBiochimica et Biophysica Acta - General Subjects
Volume1820
Issue number7
DOIs
StatePublished - Jul 1 2012
Externally publishedYes

Fingerprint

Crystallins
Oxidative stress
Cytochromes c
Lenses
Oxidative Stress
Cells
Mitochondria
Mitochondrial Membrane Potential
Oxidation
Membranes
Crystalline Lens
Energy Transfer
Macular Degeneration
Immunoprecipitation
Methionine
Energy transfer
Cataract

Keywords

  • αB-crystallin
  • Cytochrome c
  • Mitochondrion
  • Oxidation
  • Small heat shock protein

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

αb-crystallin/sHSP protects cytochrome c and mitochondrial function against oxidative stress in lens and retinal cells. / Estrada, Rebecca S.; Lee Kantorow, Wanda; Chauss, Daniel C.; Wei, Jianning; Brennan, Lisa A.; Kantorow, Marc.

In: Biochimica et Biophysica Acta - General Subjects, Vol. 1820, No. 7, 01.07.2012, p. 921-930.

Research output: Contribution to journalArticle

Estrada, Rebecca S. ; Lee Kantorow, Wanda ; Chauss, Daniel C. ; Wei, Jianning ; Brennan, Lisa A. ; Kantorow, Marc. / αb-crystallin/sHSP protects cytochrome c and mitochondrial function against oxidative stress in lens and retinal cells. In: Biochimica et Biophysica Acta - General Subjects. 2012 ; Vol. 1820, No. 7. pp. 921-930.
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T1 - αb-crystallin/sHSP protects cytochrome c and mitochondrial function against oxidative stress in lens and retinal cells

AU - Estrada, Rebecca S.

AU - Lee Kantorow, Wanda

AU - Chauss, Daniel C.

AU - Wei, Jianning

AU - Brennan, Lisa A.

AU - Kantorow, Marc

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N2 - Background: αB-crystallin/sHSP protects cells against oxidative stress damage. Here, we mechanistically examined its ability to preserve mitochondrial function in lens and retinal cells and protect cytochrome c under oxidative stress conditions. Methods: αB-crystallin/sHSP was localized in human lens (HLE-B3) and retinal (ARPE-19) cells. αB-crystallin/sHSP was stably over-expressed and its ability to preserve mitochondrial membrane potential under oxidative stress conditions was monitored. Interactions between αB-crystallin/sHSP and cytochrome c were examined by fluorescent resonance energy transfer (FRET) and by co-immune precipitation. The ability of αB-crystallin/sHSP to protect cytochrome c against methionine-80 oxidation was monitored. Results: αB-crystallin/sHSP is present in the mitochondria of lens and retinal cells and is translocated to the mitochondria under oxidative conditions. αB-crystallin/sHSP specifically interacts with cytochrome c in vitro and in vivo and its overexpression preserves mitochondrial membrane potential under oxidative stress conditions. αB-crystallin/sHSP directly protects cytochrome c against oxidation. General significance: These data demonstrate that αB-crystallin/sHSP maintains lens and retinal cells under oxidative stress conditions at least in part by preserving mitochondrial function and by protecting cytochrome c against oxidation. Since oxidative stress and loss of mitochondrial function are associated with eye lens cataract and age-related macular degeneration, loss of these αB-crystallin/sHSP functions likely plays a key role in the development of these diseases. αB-crystallin/sHSP is expressed throughout the body and its ability to maintain mitochondrial function is likely important for the prevention of multiple degenerative diseases.

AB - Background: αB-crystallin/sHSP protects cells against oxidative stress damage. Here, we mechanistically examined its ability to preserve mitochondrial function in lens and retinal cells and protect cytochrome c under oxidative stress conditions. Methods: αB-crystallin/sHSP was localized in human lens (HLE-B3) and retinal (ARPE-19) cells. αB-crystallin/sHSP was stably over-expressed and its ability to preserve mitochondrial membrane potential under oxidative stress conditions was monitored. Interactions between αB-crystallin/sHSP and cytochrome c were examined by fluorescent resonance energy transfer (FRET) and by co-immune precipitation. The ability of αB-crystallin/sHSP to protect cytochrome c against methionine-80 oxidation was monitored. Results: αB-crystallin/sHSP is present in the mitochondria of lens and retinal cells and is translocated to the mitochondria under oxidative conditions. αB-crystallin/sHSP specifically interacts with cytochrome c in vitro and in vivo and its overexpression preserves mitochondrial membrane potential under oxidative stress conditions. αB-crystallin/sHSP directly protects cytochrome c against oxidation. General significance: These data demonstrate that αB-crystallin/sHSP maintains lens and retinal cells under oxidative stress conditions at least in part by preserving mitochondrial function and by protecting cytochrome c against oxidation. Since oxidative stress and loss of mitochondrial function are associated with eye lens cataract and age-related macular degeneration, loss of these αB-crystallin/sHSP functions likely plays a key role in the development of these diseases. αB-crystallin/sHSP is expressed throughout the body and its ability to maintain mitochondrial function is likely important for the prevention of multiple degenerative diseases.

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KW - Cytochrome c

KW - Mitochondrion

KW - Oxidation

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