Structural biology of wobble-specific tRNA deaminases

  • Kim, Jungwook (PI)

Project: Research project

Project Details

Description

The conversion of genetically encoded adenosine (A) to inosine (I) at the wobble position of tRNA anticodons
is essential for the flow of biological information. This modification allows for a single tRNA species to
recognize multiple synonymous codons containing A, C or U in the last (3'-most) position and ensures that all
sense codons are appropriately decoded. While these enzymes catalyze one of the most critical and widely
conserved reactions in biology, a detailed understanding has been hampered by the lack of direct high
resolution structural information and the inability to perform quantitative kinetic analyses. The overall goal of
the proposed work is to develop the tools and infrastructure to define the structure-function relationships of the
prokaryotic and eukaryotic adenosine deaminases that act on tRNA (ADATs). In order to achieve these goals,
three specific aims will be addressed: 1) High resolution structural analysis of a liganded and unliganded
prokaryotic wobble-specific deaminase, 2) Detailed structural and mechanistic analysis of eukaryotic
wobble-specific deaminases, and 3) Identification of structural determinants in a different substrate recognition
between bacterial and eukaryotic ADAT.
StatusFinished
Effective start/end date2/1/065/31/08

Funding

  • National Institute of General Medical Sciences: $45,976.00
  • National Institute of General Medical Sciences: $17,976.00
  • National Institute of General Medical Sciences: $48,796.00

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