SPONTANEOUS MUTATIONS IN A LACZ TRANSGENE AND IN RELATION TO DEVELOPMENT &AGING

  • Vijg, Jan (PI)
  • Gwathmey, Judith (PI)
  • Wei, Jeanne (PI)
  • Boerrigter, Michael (PI)
  • Ingwall, Joanne (PI)
  • Vijg, Jan (PI)

Project: Research project

Project Details

Description

It has been hypothesized that somatic mutations are a major causal factor
in aging and age-related diseases such as cancer and atherosclerosis.
Recently, shuttle vector-based transgenic mouse models have been
developed that allow accurate quantitation and characterization of
mutations in vivo. The broad long-term objective of this proposal is to
use these mouse models to obtain more insight in mutation frequency and
their mechanism of induction in relation to development and in vivo
aging. The specific aim of this project is to determine spontaneous and
induced mutation spectra in several organs and tissues of mice from
different transgenic strains at different age-levels including
embryogenesis. The transgenic mouse model systems used throughout this
project are different CD2 and C57B1/6-based lines harboring LacZ
mutational target genes in bacteriophage lambda and plasmid vectors.
These vectors are integrated in a head-to-tail arrangement, for each line
at a different site in the genome. Spontaneous mutation frequency will
be determined in suitable bacterial hosts using established procedures;
a representative part of the mutants obtained will be further
characterized by sequencing.
StatusFinished
Effective start/end date1/1/016/30/99

ASJC

  • Cardiology and Cardiovascular Medicine
  • Genetics
  • Molecular Biology

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