ROLE OF EPIDERMAL GROWTH FACTOR RECEPTOR IN ENTEROCYTES

Project: Research project

Project Details

Description

Epidermal Growth Factor, (EGF), which is present in the
intestinal lumen from breast milk and saliva, influences intestinal
differentiation, although the mechanisms by which these effects
are achieved remain obscure. Preliminary data demonstrate the
presence of specific EGF receptors and tyrosine kinase activity in
fetal and adult rat intestinal microvillus membrane (MVM). The
proposed studies will test the hypothesis that luminal EGF
enhances intestinal proliferation by binding to villus MVM
receptors which modulate MVM phosphorylation and
transepithelial transport of EGF for subsequent interaction with
proliferating crypt cells, presumably at basolateral membrane
(BLM) receptors. Evidence for this hypothesis will be obtained
using morphological and biochemical techniques, including
autoradiography, LM and EM immunocytochemistry, (125I)-EGF
binding to specific membrane domains (MVM and BLM) and
immunoprecipitation of labeled EGF receptor from MVM. To
ascertain the role of both MVM and BLM EGF receptors in signal
transduction, tyrosine kinase activity and substrate specificity
will be studied in each membrane domain as well as in intact
intestine after exposure to both luminal and serosal EGF.
StatusFinished
Effective start/end date1/1/901/1/90

Funding

  • National Institute of Diabetes and Digestive and Kidney Diseases

ASJC

  • Biophysics
  • Physiology
  • Biochemistry
  • Cell Biology
  • Pharmacology

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