PILOT STUDY--MICROTUBULE FUNCTION AFTER PARTIAL HEPATECTOMY

  • Reid, Lola (PI)
  • Krauter, Kenneth (PI)
  • Shafritz, David (PI)
  • Roy-Chowdhury, Jayanta (PI)
  • Rogler, Charles E. (PI)
  • Satir, Brigit (PI)
  • Backer, Jonathan (PI)
  • Horwitz, Susan (PI)
  • Goldfischer, Sidney (PI)
  • Satir, Peter (PI)
  • Meier, U. Thomas (PI)
  • Davidson, Anne (PI)
  • Backer, Jonathan M. (PI)
  • Hays, Richard (PI)
  • Rogler, Leslie (PI)
  • Roy-Chowdhury, Jayanta (PI)
  • Rogler, Charles (PI)
  • Backer, Jonathan M. (PI)
  • Rogler, Charles E. (PI)
  • Meier, U. Thomas (PI)
  • Davidson, Anne (PI)
  • Hays, Richard (PI)
  • Rogler, Leslie (PI)
  • Roy-Chowdhury, Jayanta (PI)
  • Rogler, Charles (PI)
  • Band Horwitz, Susan (PI)
  • Band Horwitz, Susan (PI)
  • Satir, Peter (PI)
  • Satir, Peter (PI)
  • Snapp, Erik L. (PI)
  • Wolkoff, Allan W. (PI)

Project: Research project

Project Details

Description

Partial hepatectomy in the rat dramatically disrupts hepatocellular
microtubule in the remnant liver, as seen by confocal microscopy of liver
sections. The resulting "microtubuleless" hepatocyte has provided us
with a unique in vivo system, in which a variety of studies can be
carried out. We will examine the mechanism of microtubule disruption,
including the role of cell cycle-specific gene products and the effects
of hormones and growth factors. We will also determine the effect of
microtubule disruption on 1) intracellular transport and sorting of
vesicles, a process normally dependent on intact microtubule, and 2) gene
targeting, which is greatly facilitated by partial hepatectomy and
possibly by the concurrent loss of tubules. These studies, which employ
the unique "optical sectioning" capability of the laser scanning confocal
microscope, have the advantage of utilizing native hepatocellular tissue
rather than cells in culture, permitting us to observe events in a truly
physiologic setting.
StatusActive
Effective start/end date1/1/015/31/21

Funding

  • National Institute of Diabetes and Digestive and Kidney Diseases: $665,559.00
  • National Institute of Diabetes and Digestive and Kidney Diseases: $939,375.00
  • National Institute of Diabetes and Digestive and Kidney Diseases: $542,609.00
  • National Institutes of Health
  • National Institute of Diabetes and Digestive and Kidney Diseases: $1,252,500.00
  • National Institute of Diabetes and Digestive and Kidney Diseases: $313,125.00
  • National Institute of Diabetes and Digestive and Kidney Diseases
  • National Institute of Diabetes and Digestive and Kidney Diseases: $1,252,500.00
  • National Institute of Diabetes and Digestive and Kidney Diseases
  • National Institute of Diabetes and Digestive and Kidney Diseases

ASJC

  • Gastroenterology
  • Genetics
  • Molecular Biology
  • Structural Biology
  • Biochemistry
  • Oncology
  • Biotechnology
  • Catalysis
  • Cell Biology
  • Hepatology
  • Immunology
  • Nephrology
  • Medicine(all)
  • Atomic and Molecular Physics, and Optics
  • Biochemistry, Genetics and Molecular Biology(all)
  • Cancer Research
  • Filtration and Separation

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