HEPATIC ORGANIC ANION UPTAKE AND TRANSPARENT

Project: Research project

Project Details

Description

Uptake of organic anions by the liver has kinetic characteristics of
carrier-mediated transport. The nature of the carrier and the relationship
of transmembrane transport to specific cellular events such as plasma
membrane binding, cellular proliferation and cytoskeletal interactions are
unknown. In previous work, we studied the interaction of organic anions
with purified rat liver cell plasma membrane (LPM), and identified and
purified an organic anion binding protein (OABP) from a sinusoidal enriched
LPM subfraction. We also defined transient selective reduction in organic
anion influx in regenerating liver, liver from rats treated with nafenopin
and liver from fasted rats. Influx, as quantitated by the methods we
employ is independent of hepatic mass, represents specific interaction of
ligand with LPM and is not influenced by intracellular events, such as
protein binding or metabolism. The long-range goal is to understand the
mechanism of transmembrane organic anion movement on normal liver and in
various acquired and inheritable disorders. The specific aims are: (1) To
quantitate immunologically OABP in liver the function of OABP in hepatic
organic anion transport. (3) To compare bilirubin and BSP binding to LPM
from normal, partially hepatectomized, fasted or nafenopin-treated rats.
(4) To determine whether bilirubin and BSP enter the hepatocyte by a single
mechanism or by overlapping, but partially independent mechanisms. (5) To
study the role of microtubules and microfilaments in transmembrane movement
of organic anions in normal and regenerating liver.
StatusFinished
Effective start/end date3/1/792/28/87

Funding

  • National Institutes of Health

ASJC

  • Medicine(all)
  • Biophysics
  • Physiology
  • Biochemistry
  • Environmental Chemistry
  • Hepatology

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