GUANINE NUCLEOTIDE BINDING PROTEINS AS RECEPTOR-EFFECTOR COUPLERS

Project: Research project

Project Details

Description

A family of guanine nucleotide binding proteins (G-proteins)
functions in transmembrane signalling as receptor-effector
couplers. G-proteins couple to a diverse array of receptors
including those for hormones, neurotransmitters, light, odorants,
and certain growth factors. Effector functions regulated
(positively and, in some instances, negatively) by G-proteins
include cAMP formation, phosphoinositide breakdown, potassium and
calcium channels, and cGMP degradation. We have used a variety of
techniques to study the expression, distribution, regulation,
structure and function of G-proteins. Our studies highlight the
diversity within the G-protein family. We have purified novel G-
proteins and using cloned cDNAs, defined their primary structure
and distribution. We have demonstrated developmental and
differentiation-dependent regulation of G-protein synthesis. Using
peptide specific antibodies, in situ hybridization and northern
analyses, and protein reconstitution techniques, we have defined
the specificity of G-proteins in coupling to receptors and
effectors. We have cloned and characterized the human gene for a
G-protein to define the basis for regulation of expression. These
studies provide the basis for understanding the role of G-proteins
in normal signal transduction and for elucidating possible defects
in G-protein structure or function as the basis for abnormal signal
transduction.
StatusNot started

ASJC

  • Cell Biology