AIDA-1 in APP metabolism and Synaptic Function

Project: Research project

Description

DESCRIPTION (provided by applicant): Deposition of beta-amyloid peptide (A2) within neuritic plaques is a hallmark pathology of Alzheimer's disease (AD). Processing of the Amyloid Precursor Protein (APP) by 2 and 3-secretases results in the generation of A2 peptides. Excess A2 is believed to be a main contributor to the dysfunction and degeneration of neurons that underlies AD. As such, preventing A2 deposition or clearing excess pathogenic A2 remains an important therapeutic target in AD. APP internalization is required for A2 production and there is evidence that general endocytic dysregulations underlie sporadic AD and AD- like pathophysiologies. Therefore a better understanding of the mechanisms that regulate APP internalization would guide more selective strategies for developing AD therapies. In this proposal we will use biochemical, cell biology and imaging analysis of primary neuronal cultures to test the hypothesis that the novel APP adaptor AIDA-1 regulates A2 production by promoting APP internalization. To determine if AIDA-1 regulates APP metabolism in vivo we will quantify APP proteolytic fragments in newly generated AIDA-1 knockout mice, and determine if loss of AIDA-1 can rescue AD-like phenotypes in mouse models of AD. Moreover we will evaluate the therapeutic potential of cell-permeable peptides that block AIDA-1/APP interactions by determining their effects on excess A2 production in AD mouse models. We will also determine how loss of AIDA-1 affects synaptic function in cell cultures and in acute hippocampal slices from AIDA-1 knockout mice and determine if loss of AIDA-1 can revert the synaptic deficits observed in AD mouse models. With the tools developed here, we will be able to address the chronic and acute effects of loss of AIDA-1 and endogenous A2 production on neurotransmission. This is important given AD has been increasingly viewed as a synaptic dysfunction caused by excess A2 production. PUBLIC HEALTH RELEVANCE: Alzheimer's disease (AD) is the 7th leading cause of death in the United States, with an estimated 5.3 million diseased individuals, resulting in ~172 billion dollars in annual costs (Alzheimer's association 2010 report). Excess beta-amyloid peptides (A2), either soluble or deposited in neuritic plaques are believed to be a main contributor to the dysfunction and degeneration of neurons that occurs in AD. In this proposal we will characterize the role of the novel synaptic component AIDA-1 in the generation of A2 peptides, determine the therapeutic potential of inhibiting AIDA-1 function and determine the effects of AIDA-1 on synaptic transmission.
StatusFinished
Effective start/end date8/1/115/31/17

Funding

  • National Institutes of Health: $259,085.00
  • National Institutes of Health: $340,300.00
  • National Institutes of Health: $71,006.00
  • National Institutes of Health: $340,300.00
  • National Institutes of Health: $340,300.00
  • National Institutes of Health: $321,584.00

Fingerprint

Amyloid beta-Protein Precursor
Alzheimer Disease
varespladib methyl
Knockout Mice
Nerve Degeneration
Peptides
Amyloid Plaques
Amyloid Precursor Protein Secretases
Synaptic Transmission
Amyloid beta-Peptides
Cell Biology
Therapeutics
Cell Culture Techniques
Pathology
Phenotype

ASJC

  • Medicine(all)