Three-dimensional dynamics of pseudopod formation and the regulation of turning during the motility cycle of Dictyostelium

D. Wessels, H. Vawter-Hugart, John W. Murray, D. R. Soll

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

Employing a newly developed computer-assisted system for visualizing and quantitating cell motility in three dimensions, we have examined the 3- dimensional changes in cell shape and the dynamics of pseudopod extension during translocation of Dictyostelium amoebae. Amoebae exhibit a 3- dimensional behavior cycle with an average period of 1.5 min. The cycle includes a transient pseudopod extension phase in the x,y axis followed by a z-axis expansion phase. Anterior pseudopod extension in the x,y axis is accompanied by a decrease in height, not by uropod retraction. The increase in height is accompanied by uropod retraction. In the pseudopod extension phase in the x,y axes, pseudopods form either anteriorly or laterally, and either on or above the substratum. Pseudopods which initially form on the substratum in almost all cases continue to expand as the anterior end of the cell. In the case of lateral pseudopods, anteriorization leads to a turn. Approximately half of anterior pseudopod and two-thirds of lateral pseudopods which initially form above the substratum are retracted. These results suggest that pseudopod-substratum interaction plays a fundamental role in the regulation of directionality and turning in the translocation phase of the 3- dimensional behavior cycle.

Original languageEnglish (US)
Pages (from-to)1-12
Number of pages12
JournalCell Motility and the Cytoskeleton
Volume27
Issue number1
StatePublished - 1994
Externally publishedYes

Fingerprint

Pseudopodia
Dictyostelium
Amoeba
Cell Shape
Computer Systems
Cell Movement

Keywords

  • amoebae
  • pseudopod extension
  • uropod retraction

ASJC Scopus subject areas

  • Cell Biology

Cite this

Three-dimensional dynamics of pseudopod formation and the regulation of turning during the motility cycle of Dictyostelium. / Wessels, D.; Vawter-Hugart, H.; Murray, John W.; Soll, D. R.

In: Cell Motility and the Cytoskeleton, Vol. 27, No. 1, 1994, p. 1-12.

Research output: Contribution to journalArticle

@article{effa2fe822524fd3842e972497e84cfa,
title = "Three-dimensional dynamics of pseudopod formation and the regulation of turning during the motility cycle of Dictyostelium",
abstract = "Employing a newly developed computer-assisted system for visualizing and quantitating cell motility in three dimensions, we have examined the 3- dimensional changes in cell shape and the dynamics of pseudopod extension during translocation of Dictyostelium amoebae. Amoebae exhibit a 3- dimensional behavior cycle with an average period of 1.5 min. The cycle includes a transient pseudopod extension phase in the x,y axis followed by a z-axis expansion phase. Anterior pseudopod extension in the x,y axis is accompanied by a decrease in height, not by uropod retraction. The increase in height is accompanied by uropod retraction. In the pseudopod extension phase in the x,y axes, pseudopods form either anteriorly or laterally, and either on or above the substratum. Pseudopods which initially form on the substratum in almost all cases continue to expand as the anterior end of the cell. In the case of lateral pseudopods, anteriorization leads to a turn. Approximately half of anterior pseudopod and two-thirds of lateral pseudopods which initially form above the substratum are retracted. These results suggest that pseudopod-substratum interaction plays a fundamental role in the regulation of directionality and turning in the translocation phase of the 3- dimensional behavior cycle.",
keywords = "amoebae, pseudopod extension, uropod retraction",
author = "D. Wessels and H. Vawter-Hugart and Murray, {John W.} and Soll, {D. R.}",
year = "1994",
language = "English (US)",
volume = "27",
pages = "1--12",
journal = "Cytoskeleton",
issn = "1949-3584",
publisher = "Wiley-Liss Inc.",
number = "1",

}

TY - JOUR

T1 - Three-dimensional dynamics of pseudopod formation and the regulation of turning during the motility cycle of Dictyostelium

AU - Wessels, D.

AU - Vawter-Hugart, H.

AU - Murray, John W.

AU - Soll, D. R.

PY - 1994

Y1 - 1994

N2 - Employing a newly developed computer-assisted system for visualizing and quantitating cell motility in three dimensions, we have examined the 3- dimensional changes in cell shape and the dynamics of pseudopod extension during translocation of Dictyostelium amoebae. Amoebae exhibit a 3- dimensional behavior cycle with an average period of 1.5 min. The cycle includes a transient pseudopod extension phase in the x,y axis followed by a z-axis expansion phase. Anterior pseudopod extension in the x,y axis is accompanied by a decrease in height, not by uropod retraction. The increase in height is accompanied by uropod retraction. In the pseudopod extension phase in the x,y axes, pseudopods form either anteriorly or laterally, and either on or above the substratum. Pseudopods which initially form on the substratum in almost all cases continue to expand as the anterior end of the cell. In the case of lateral pseudopods, anteriorization leads to a turn. Approximately half of anterior pseudopod and two-thirds of lateral pseudopods which initially form above the substratum are retracted. These results suggest that pseudopod-substratum interaction plays a fundamental role in the regulation of directionality and turning in the translocation phase of the 3- dimensional behavior cycle.

AB - Employing a newly developed computer-assisted system for visualizing and quantitating cell motility in three dimensions, we have examined the 3- dimensional changes in cell shape and the dynamics of pseudopod extension during translocation of Dictyostelium amoebae. Amoebae exhibit a 3- dimensional behavior cycle with an average period of 1.5 min. The cycle includes a transient pseudopod extension phase in the x,y axis followed by a z-axis expansion phase. Anterior pseudopod extension in the x,y axis is accompanied by a decrease in height, not by uropod retraction. The increase in height is accompanied by uropod retraction. In the pseudopod extension phase in the x,y axes, pseudopods form either anteriorly or laterally, and either on or above the substratum. Pseudopods which initially form on the substratum in almost all cases continue to expand as the anterior end of the cell. In the case of lateral pseudopods, anteriorization leads to a turn. Approximately half of anterior pseudopod and two-thirds of lateral pseudopods which initially form above the substratum are retracted. These results suggest that pseudopod-substratum interaction plays a fundamental role in the regulation of directionality and turning in the translocation phase of the 3- dimensional behavior cycle.

KW - amoebae

KW - pseudopod extension

KW - uropod retraction

UR - http://www.scopus.com/inward/record.url?scp=0028127742&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028127742&partnerID=8YFLogxK

M3 - Article

VL - 27

SP - 1

EP - 12

JO - Cytoskeleton

JF - Cytoskeleton

SN - 1949-3584

IS - 1

ER -