The water permeability of toad urinary bladder. II. The value of P(f)/P(d)(w) for the antidiuretic hormone-induced water permeation pathway

S. D. Levine, M. Jacoby, A. Finkelstein

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Abstract

Using the methods described in the preceding paper (Levine et al., 1984) for measuring the magnitude of the water-permeable barriers in series with the luminal membrane, we correct measured values of P(d)(w) in bladders stimulated with low doses of antidiuretic hormone (ADH) or 8-bromo cyclic AMP to obtain their true values in the luminal membrane. Simultaneously, we also determine P(f). We thus are able to calculate P(f)/P(d)(w) for the hormone-induced water permeation pathway in the luminal membrane. Our finding is that P(f)/P(d)(w) ~ 17. Two channel models consistent both with this value and the impermeability of the ADH-induced water permeation pathway to small nonelectrolytes are: (a) a long (~50 Å), small-radius (~2 Å) pore through which 17 water molecules pass in single-file array, and (b) a shower-head-like structure in which the stem is long and of large radius (~20 Å) and the cap has numerous short, small-radius (~2 Å) pores. A third possibility is that whereas the selective permeability to H2O results from small-radius (~2 Å) pores, the large value of P(f)/P(d)(w) arises from their location in the walls of long tubular vesicles (~2 μm in length and 0.1 μm in diameter) that are functionally part of the luminal membrane after having fused with it. Aggregate-containing tubular vesicles of these dimensions have been reported to fuse with the luminal membrane in response to ADH stimulation and have been implicated in the ADH-induced hydroosmotic response.

Original languageEnglish (US)
Pages (from-to)543-561
Number of pages19
JournalJournal of General Physiology
Volume83
Issue number4
StatePublished - 1984

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Vasopressins
Anura
Permeability
Urinary Bladder
Membranes
Water
8-Bromo Cyclic Adenosine Monophosphate
Head
Hormones

ASJC Scopus subject areas

  • Physiology

Cite this

The water permeability of toad urinary bladder. II. The value of P(f)/P(d)(w) for the antidiuretic hormone-induced water permeation pathway. / Levine, S. D.; Jacoby, M.; Finkelstein, A.

In: Journal of General Physiology, Vol. 83, No. 4, 1984, p. 543-561.

Research output: Contribution to journalArticle

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abstract = "Using the methods described in the preceding paper (Levine et al., 1984) for measuring the magnitude of the water-permeable barriers in series with the luminal membrane, we correct measured values of P(d)(w) in bladders stimulated with low doses of antidiuretic hormone (ADH) or 8-bromo cyclic AMP to obtain their true values in the luminal membrane. Simultaneously, we also determine P(f). We thus are able to calculate P(f)/P(d)(w) for the hormone-induced water permeation pathway in the luminal membrane. Our finding is that P(f)/P(d)(w) ~ 17. Two channel models consistent both with this value and the impermeability of the ADH-induced water permeation pathway to small nonelectrolytes are: (a) a long (~50 {\AA}), small-radius (~2 {\AA}) pore through which 17 water molecules pass in single-file array, and (b) a shower-head-like structure in which the stem is long and of large radius (~20 {\AA}) and the cap has numerous short, small-radius (~2 {\AA}) pores. A third possibility is that whereas the selective permeability to H2O results from small-radius (~2 {\AA}) pores, the large value of P(f)/P(d)(w) arises from their location in the walls of long tubular vesicles (~2 μm in length and 0.1 μm in diameter) that are functionally part of the luminal membrane after having fused with it. Aggregate-containing tubular vesicles of these dimensions have been reported to fuse with the luminal membrane in response to ADH stimulation and have been implicated in the ADH-induced hydroosmotic response.",
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