13C NMR of the bases of three DNA oligonucleotide duplexes

Assignment methods and structural features

Steven R. LaPlante, Eilis A. Boudreau, Nilo Zanatta, George C. Levy, Philip N. Borer, Joseph Ashcroft, David Cowburn

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Natural abundance 13C NMR spectra of three DNA oligomers have been obtained. Most of the base resonances are well resolved from one another. A combination of two independent methods was used in making assignments: a one-dimensional spectral comparison method and a two-dimensional proton-detected 1H-13C correlated experiment for the protonated carbons. There are large shielding changes (between 1.62 and -1.40 ppm) upon thermal dissociation of the duplex. The shapes of the chemical shift vs temperature curves are largely independent of sequence. The base carbon resonance frequencies are sensitive to hydrogen bonding, base stacking, sugar conformation, and changes in the glycosyl torsion angle.

Original languageEnglish (US)
Pages (from-to)7902-7909
Number of pages8
JournalBiochemistry
Volume27
Issue number20
StatePublished - 1988
Externally publishedYes

Fingerprint

Oligonucleotides
Carbon
Nuclear magnetic resonance
DNA
Chemical shift
Hydrogen Bonding
Oligomers
Sugars
Shielding
Torsional stress
Conformations
Protons
Hydrogen bonds
Hot Temperature
Temperature
Experiments
Carbon-13 Magnetic Resonance Spectroscopy

ASJC Scopus subject areas

  • Biochemistry

Cite this

LaPlante, S. R., Boudreau, E. A., Zanatta, N., Levy, G. C., Borer, P. N., Ashcroft, J., & Cowburn, D. (1988). 13C NMR of the bases of three DNA oligonucleotide duplexes: Assignment methods and structural features. Biochemistry, 27(20), 7902-7909.

13C NMR of the bases of three DNA oligonucleotide duplexes : Assignment methods and structural features. / LaPlante, Steven R.; Boudreau, Eilis A.; Zanatta, Nilo; Levy, George C.; Borer, Philip N.; Ashcroft, Joseph; Cowburn, David.

In: Biochemistry, Vol. 27, No. 20, 1988, p. 7902-7909.

Research output: Contribution to journalArticle

LaPlante, SR, Boudreau, EA, Zanatta, N, Levy, GC, Borer, PN, Ashcroft, J & Cowburn, D 1988, '13C NMR of the bases of three DNA oligonucleotide duplexes: Assignment methods and structural features', Biochemistry, vol. 27, no. 20, pp. 7902-7909.
LaPlante SR, Boudreau EA, Zanatta N, Levy GC, Borer PN, Ashcroft J et al. 13C NMR of the bases of three DNA oligonucleotide duplexes: Assignment methods and structural features. Biochemistry. 1988;27(20):7902-7909.
LaPlante, Steven R. ; Boudreau, Eilis A. ; Zanatta, Nilo ; Levy, George C. ; Borer, Philip N. ; Ashcroft, Joseph ; Cowburn, David. / 13C NMR of the bases of three DNA oligonucleotide duplexes : Assignment methods and structural features. In: Biochemistry. 1988 ; Vol. 27, No. 20. pp. 7902-7909.
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