Protein-protein binding supersites

Raji Viswanathan, Jorge E. Fajardo, Gabriel Steinberg, Matthew Haller, Andras Fiser

Research output: Contribution to journalArticle

Abstract

The lack of a deep understanding of how proteins interact remains an important roadblock in advancing efforts to identify binding partners and uncover the corresponding regulatory mechanisms of the functions they mediate. Understanding protein-protein interactions is also essential for designing specific chemical modifications to develop new reagents and therapeutics. We explored the hypothesis of whether protein interaction sites serve as generic biding sites for non-cognate protein ligands, just as it has been observed for small-molecule-binding sites in the past. Using extensive computational docking experiments on a test set of 241 protein complexes, we found that indeed there is a strong preference for non-cognate ligands to bind to the cognate binding site of a receptor. This observation appears to be robust to variations in docking programs, types of non-cognate protein probes, sizes of binding patches, relative sizes of binding patches and full-length proteins, and the exploration of obligate and non-obligate complexes. The accuracy of the docking scoring function appears to play a role in defining the correct site. The frequency of interaction of unrelated probes recognizing the binding interface was utilized in a simple prediction algorithm that showed accuracy competitive with other state of the art methods.

Original languageEnglish (US)
Pages (from-to)e1006704
JournalPLoS Computational Biology
Volume15
Issue number1
DOIs
StatePublished - Jan 1 2019

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protein binding
Protein Binding
Proteins
Protein
protein
Docking
proteins
Patch
binding sites
Probe
Binding sites
ligand
Binding Sites
Ligands
probe
protein-protein interactions
Protein-protein Interaction
Test Set
Scoring
Interaction

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Modeling and Simulation
  • Ecology
  • Molecular Biology
  • Genetics
  • Cellular and Molecular Neuroscience
  • Computational Theory and Mathematics

Cite this

Protein-protein binding supersites. / Viswanathan, Raji; Fajardo, Jorge E.; Steinberg, Gabriel; Haller, Matthew; Fiser, Andras.

In: PLoS Computational Biology, Vol. 15, No. 1, 01.01.2019, p. e1006704.

Research output: Contribution to journalArticle

Viswanathan, Raji ; Fajardo, Jorge E. ; Steinberg, Gabriel ; Haller, Matthew ; Fiser, Andras. / Protein-protein binding supersites. In: PLoS Computational Biology. 2019 ; Vol. 15, No. 1. pp. e1006704.
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