Interleukin 1 and tumor necrosis factor-α stimulate the production of colony-stimulating factor 1 by murine astrocytes

Clotilde Théry, E. Richard Stanley, Michel Mallat

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Astrocytes have the ability to secrete colony-stimulating factor 1 (CSF-1), a growth factor known to stimulate the proliferation of brain macrophages. We have studied the effect of cytokines such as interleukin 1 (IL-1), tumor necrosis factor-α (TNFα), and interleukin 6 (IL-6) on the production of CSF-1 by cultured primary astrocytes and an astrocytic cell line derived from embryonic mouse brain. We observed that both TNFα and IL-1 increased CSF-1 mRNA and protein levels in the astrocytic cultures. In contrast, IL-6 was ineffective. The CSF-1 mRNA levels were strongly reduced by incubating immortalized astrocytic cells with stauro-sporine, a protein kinase C inhibitor, both in the absence and in the presence of cytokines. Conversely, 12-O-tetradecanoylphorbol 13-acetate, a protein kinase C activator, increased CSF-1 mRNA levels. These results suggest a mechanism whereby mononuclear phagocytes could favor their own recruitment in the CNS by producing cytokines.

Original languageEnglish (US)
Pages (from-to)1183-1186
Number of pages4
JournalJournal of Neurochemistry
Volume59
Issue number3
StatePublished - Sep 1992

Fingerprint

Macrophage Colony-Stimulating Factor
Interleukin-1
Astrocytes
Tumor Necrosis Factor-alpha
Cytokines
Messenger RNA
Protein Kinase C
Interleukin-6
Brain
Protein C Inhibitor
Macrophages
Tetradecanoylphorbol Acetate
Protein Kinase Inhibitors
Phagocytes
Intercellular Signaling Peptides and Proteins
Acetates
Cells
Cell Line
Proteins

Keywords

  • Astrocytes
  • Colony-stimulating factor 1
  • Interleukin 1
  • Interleukin 6
  • Tumor necrosis factor-α

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Interleukin 1 and tumor necrosis factor-α stimulate the production of colony-stimulating factor 1 by murine astrocytes. / Théry, Clotilde; Stanley, E. Richard; Mallat, Michel.

In: Journal of Neurochemistry, Vol. 59, No. 3, 09.1992, p. 1183-1186.

Research output: Contribution to journalArticle

Théry, Clotilde ; Stanley, E. Richard ; Mallat, Michel. / Interleukin 1 and tumor necrosis factor-α stimulate the production of colony-stimulating factor 1 by murine astrocytes. In: Journal of Neurochemistry. 1992 ; Vol. 59, No. 3. pp. 1183-1186.
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