Developmental regulation of mRNAs encoding rat brain kainate/AMPA receptors

A northern analysis study

Guylaine M. Durand, R. Suzanne Zukin

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

Functionally diverse kainate/α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors are generated by assembly of glutamate receptor (GluR)1, 2, and 3 subunits into homomeric and heteromeric channels. We examined GluR1, 2, and 3 gene expression in embryonic, neonatal, and adult rat brain by northern analysis under conditions of high stringency. In the adult, hybridization to a GluR1 riboprobe revealed the presence of an abundant RNA species, 5.2 kb in size, and minor bands of 3.2 and 3.9 kb. GluR2 hybridized to two species, 3.9 and 5.9 kb, of comparable abundance, presumably attributable to alternate splice products. Hybridization to the GluRS riboprobe showed a major species of 5.2 kb. This pattern of RNA species was invariant over all the brain regions examined. Examination of GluR expression in development revealed that in the postnatal period, GluR1, 2, and 3 mRNAs are regulated as a function of age. In adult rat brain, GluR1 and 2 mRNA expression was highest in hippocampus; G1uRS was expressed at highest density in hippocampus and frontal cortex. The three transcripts were first detected at embryonic day 16 and then exhibited changes in expression levels in a region-specific manner. In hippocampus, all three transcripts exhibited elevated expression in the late neonatal period; in frontal cortex, elevated expression was observed for GluR2 and 3 only. In striatum, all three transcripts were expressed at relatively low levels throughout development, with a modest peak at postnatal day 14. In cerebellum, the GluR1 mRNA level was high from postnatal day 28 to adult. Studies of recombinant receptors indicate that GluR1 and 3 subunits form channels that are Ca2+ permeable; heteromeric receptors containing the GluR2 subunit are Ca2+ impermeable. These observations raise the possibility that a modification of GluR2 expression during development may regulate the level of Ca2+-permeable kainate channels. We therefore investigated the expression of the GluR2 subunit relative to those of the GluR1 and 3 subunits as a function of age. In hippocampus and frontal cortex, the (GluR1 + GluR3)/GluR2 ratio declined with increasing age. This result provides evidence for a developmental regulation of kainate/AMPA receptor gene expression in the brain during the first 2 weeks of postnatal life and predicts a decrease in glutamate-operated Ca2+ permeability mediated through kainate/AMPA receptors as a function of age.

Original languageEnglish (US)
Pages (from-to)2239-2246
Number of pages8
JournalJournal of Neurochemistry
Volume61
Issue number6
StatePublished - Dec 1993

Fingerprint

Kainic Acid Receptors
AMPA Receptors
Kainic Acid
Rats
Hippocampus
Brain
Frontal Lobe
Messenger RNA
Glutamate Receptors
Gene expression
RNA
Gene Expression
Cerebellum
Glutamic Acid
Permeability
Acids

Keywords

  • Excitatory amino acid receptors
  • Gene expression
  • Glutamate receptors

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Developmental regulation of mRNAs encoding rat brain kainate/AMPA receptors : A northern analysis study. / Durand, Guylaine M.; Zukin, R. Suzanne.

In: Journal of Neurochemistry, Vol. 61, No. 6, 12.1993, p. 2239-2246.

Research output: Contribution to journalArticle

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abstract = "Functionally diverse kainate/α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors are generated by assembly of glutamate receptor (GluR)1, 2, and 3 subunits into homomeric and heteromeric channels. We examined GluR1, 2, and 3 gene expression in embryonic, neonatal, and adult rat brain by northern analysis under conditions of high stringency. In the adult, hybridization to a GluR1 riboprobe revealed the presence of an abundant RNA species, 5.2 kb in size, and minor bands of 3.2 and 3.9 kb. GluR2 hybridized to two species, 3.9 and 5.9 kb, of comparable abundance, presumably attributable to alternate splice products. Hybridization to the GluRS riboprobe showed a major species of 5.2 kb. This pattern of RNA species was invariant over all the brain regions examined. Examination of GluR expression in development revealed that in the postnatal period, GluR1, 2, and 3 mRNAs are regulated as a function of age. In adult rat brain, GluR1 and 2 mRNA expression was highest in hippocampus; G1uRS was expressed at highest density in hippocampus and frontal cortex. The three transcripts were first detected at embryonic day 16 and then exhibited changes in expression levels in a region-specific manner. In hippocampus, all three transcripts exhibited elevated expression in the late neonatal period; in frontal cortex, elevated expression was observed for GluR2 and 3 only. In striatum, all three transcripts were expressed at relatively low levels throughout development, with a modest peak at postnatal day 14. In cerebellum, the GluR1 mRNA level was high from postnatal day 28 to adult. Studies of recombinant receptors indicate that GluR1 and 3 subunits form channels that are Ca2+ permeable; heteromeric receptors containing the GluR2 subunit are Ca2+ impermeable. These observations raise the possibility that a modification of GluR2 expression during development may regulate the level of Ca2+-permeable kainate channels. We therefore investigated the expression of the GluR2 subunit relative to those of the GluR1 and 3 subunits as a function of age. In hippocampus and frontal cortex, the (GluR1 + GluR3)/GluR2 ratio declined with increasing age. This result provides evidence for a developmental regulation of kainate/AMPA receptor gene expression in the brain during the first 2 weeks of postnatal life and predicts a decrease in glutamate-operated Ca2+ permeability mediated through kainate/AMPA receptors as a function of age.",
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