A novel CSF-1 binding factor in a patient in complete remission following cytotoxic therapy for lymphoma

A. H. Baker, P. G. Cachia, G. B. Tennant, J. A. Whittaker, D. White, E. R. Stanley, A. K. Burnett, R. A. Padua

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A novel colony stimulating factor-1 (CSF-1) binding factor present in the serum from a patient in remission from lymphoma is described. Radioimmunoassay (RIA) repeatedly failed to detect circulating levels of CSF-1 in the peripheral blood system of this patient. Molecular analysis showed a normal CSF-1 gene structure by Southern blot analysis and a 46,XX karyotype by cytogenetic analysis. CSF-1 mRNA expression in peripheral blood leucocytes was confirmed using reverse transcriptase polymerase chain reaction analysis. Morphological analysis of bone marrow cells was normal and peripheral blood progenitor cell colony assays showed a pattern of growth within the normal range in response to CSF-1 alone and in combination with other cytokines. Analysis of the patient's plasma and conditioned media prepared from peripheral blood mononuclear and granulocytic cell fractions for their ability to bind 125Iodine-labelled CSF-1 revealed the presence of a plasma CSF-1 binding factor. This binding factor was not present in the patient's urine, because CSF-1 was detected by RIA and production of the binding factor by the patients peripheral blood white cells could not be demonstrated in vitro. To our knowledge, this is the first reported case of a soluble CSF-1 binding factor.

Original languageEnglish (US)
Pages (from-to)219-222
Number of pages4
JournalBritish Journal of Haematology
Volume89
Issue number1
StatePublished - 1995
Externally publishedYes

Fingerprint

Macrophage Colony-Stimulating Factor
Lymphoma
Therapeutics
Radioimmunoassay
Leukocytes
Cytogenetic Analysis
Conditioned Culture Medium
Southern Blotting
Reverse Transcriptase Polymerase Chain Reaction
Karyotype
Bone Marrow Cells
Blood Cells
Reference Values
Stem Cells
Urine
Cytokines
Messenger RNA

Keywords

  • binding factor
  • CSF-1
  • FMS
  • lymphoma
  • serum

ASJC Scopus subject areas

  • Hematology

Cite this

Baker, A. H., Cachia, P. G., Tennant, G. B., Whittaker, J. A., White, D., Stanley, E. R., ... Padua, R. A. (1995). A novel CSF-1 binding factor in a patient in complete remission following cytotoxic therapy for lymphoma. British Journal of Haematology, 89(1), 219-222.

A novel CSF-1 binding factor in a patient in complete remission following cytotoxic therapy for lymphoma. / Baker, A. H.; Cachia, P. G.; Tennant, G. B.; Whittaker, J. A.; White, D.; Stanley, E. R.; Burnett, A. K.; Padua, R. A.

In: British Journal of Haematology, Vol. 89, No. 1, 1995, p. 219-222.

Research output: Contribution to journalArticle

Baker, AH, Cachia, PG, Tennant, GB, Whittaker, JA, White, D, Stanley, ER, Burnett, AK & Padua, RA 1995, 'A novel CSF-1 binding factor in a patient in complete remission following cytotoxic therapy for lymphoma', British Journal of Haematology, vol. 89, no. 1, pp. 219-222.
Baker AH, Cachia PG, Tennant GB, Whittaker JA, White D, Stanley ER et al. A novel CSF-1 binding factor in a patient in complete remission following cytotoxic therapy for lymphoma. British Journal of Haematology. 1995;89(1):219-222.
Baker, A. H. ; Cachia, P. G. ; Tennant, G. B. ; Whittaker, J. A. ; White, D. ; Stanley, E. R. ; Burnett, A. K. ; Padua, R. A. / A novel CSF-1 binding factor in a patient in complete remission following cytotoxic therapy for lymphoma. In: British Journal of Haematology. 1995 ; Vol. 89, No. 1. pp. 219-222.
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