Zebrafish Rho kinase 2 acts downstream of Wnt11 to mediate cell polarity and effective convergence and extension movements

Florence Marlow, Jacek Topczewski, Diane Sepich, Lilianna Solnica-Krezel

Research output: Contribution to journalArticle

252 Citations (Scopus)

Abstract

Background: During vertebrate gastrulation convergence and extension (CE), movements narrow and lengthen embryonic tissues. In Xenopus and zebrafish, a noncanonical Wnt signaling pathway constitutes the vertebrate counterpart to the Drosophila planar cell polarity pathway and regulates mediolateral cell polarization underlying CE. Despite the identification of several signaling molecules required for normal CE, the downstream transducers regulating individual cell behaviors driving CE are only beginning to be elucidated. Moreover, how defective mediolateral cell polarity impacts CE is not understood. Results: Here, we show that overexpression of zebrafish dominant-negative Rho kinase 2 (dnRok2) disrupts CE without altering cell fates, phenocopying noncanonical Wnt signaling mutants. Moreover, Rho kinase 2 (Rok2) overexpression partially suppresses the slb/wnt11 gastrulation phenotype, and ectopic expression of noncanonical Wnts modulates Rok2 intracellular distribution. In addition, time-lapse analyses associate defective dorsal convergence movements with impaired cell elongation, mediolateral orientation, and consequently failure to migrate along straight paths. Transplantation experiments reveal that dnRok2 cells in wildtype hosts neither elongate nor orient their axes. In contrast, wild-type cells are able to elongate their cell bodies in dnRok2 hosts, even though they fail to orient their axes. Conclusions: During zebrafish gastrulation, Rok2 acts downstream of noncanonical Wnt11 signaling to mediate mediolateral cell elongation required for dorsal cell movement along straight paths. Furthermore, elongation and orientation of the cell body are independent properties that require both cell-autonomous and non-autonomous Rok2 function.

Original languageEnglish (US)
Pages (from-to)876-884
Number of pages9
JournalCurrent Biology
Volume12
Issue number11
DOIs
StatePublished - Jun 4 2002
Externally publishedYes

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rho-Associated Kinases
Cell Polarity
Zebrafish
Danio rerio
phosphotransferases (kinases)
Gastrulation
Elongation
cells
Cells
Vertebrates
cell growth
vertebrates
Wnt Signaling Pathway
cell polarity
Xenopus
Transducers
cell movement
Drosophila
Cell Movement
Polarization

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Zebrafish Rho kinase 2 acts downstream of Wnt11 to mediate cell polarity and effective convergence and extension movements. / Marlow, Florence; Topczewski, Jacek; Sepich, Diane; Solnica-Krezel, Lilianna.

In: Current Biology, Vol. 12, No. 11, 04.06.2002, p. 876-884.

Research output: Contribution to journalArticle

Marlow, Florence ; Topczewski, Jacek ; Sepich, Diane ; Solnica-Krezel, Lilianna. / Zebrafish Rho kinase 2 acts downstream of Wnt11 to mediate cell polarity and effective convergence and extension movements. In: Current Biology. 2002 ; Vol. 12, No. 11. pp. 876-884.
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