YRA1 Autoregulation Requires Nuclear Export and Cytoplasmic Edc3p-Mediated Degradation of Its Pre-mRNA

Shuyun Dong, Chunfang Li, Daniel Zenklusen, Robert H. Singer, Allan Jacobson, Feng He

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Autoregulatory loops often provide precise control of the level of expression of specific genes that encode key regulatory proteins. Here we have defined a pathway by which Yra1p, a yeast mRNA export factor, controls its own expression. We show that YRA1 exon 1 sequences in cis and Yra1p in trans inhibit YRA1 pre-mRNA splicing and commit the pre-mRNA to nuclear export. Mex67p and Crm1p jointly promote YRA1 pre-mRNA export, and once in the cytoplasm, the pre-mRNA is degraded by a 5′ to 3′ decay mechanism that is dependent on the decapping activator Edc3p and on specific sequences in the YRA1 intron. These results illustrate how common steps in the nuclear processing, export, and degradation of a transcript can be uniquely combined to control the expression of a specific gene and suggest that Edc3p-mediated decay may have additional regulatory functions in eukaryotic cells.

Original languageEnglish (US)
Pages (from-to)559-573
Number of pages15
JournalMolecular Cell
Volume25
Issue number4
DOIs
StatePublished - Feb 23 2007

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Cell Nucleus Active Transport
RNA Precursors
Homeostasis
Eukaryotic Cells
Introns
Exons
Cytoplasm
Yeasts
Gene Expression
Messenger RNA
Genes
Proteins

Keywords

  • RNA

ASJC Scopus subject areas

  • Molecular Biology

Cite this

YRA1 Autoregulation Requires Nuclear Export and Cytoplasmic Edc3p-Mediated Degradation of Its Pre-mRNA. / Dong, Shuyun; Li, Chunfang; Zenklusen, Daniel; Singer, Robert H.; Jacobson, Allan; He, Feng.

In: Molecular Cell, Vol. 25, No. 4, 23.02.2007, p. 559-573.

Research output: Contribution to journalArticle

Dong, Shuyun ; Li, Chunfang ; Zenklusen, Daniel ; Singer, Robert H. ; Jacobson, Allan ; He, Feng. / YRA1 Autoregulation Requires Nuclear Export and Cytoplasmic Edc3p-Mediated Degradation of Its Pre-mRNA. In: Molecular Cell. 2007 ; Vol. 25, No. 4. pp. 559-573.
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