Yotiao protein, a ligand for the NMDA receptor, binds and targets cAMP- dependent protein kinase II

Antonio Feliciello; Luca Cardone; Corrado Garbi; Michael D. Ginsberg; Stelio Varrone; Charles S. Rubin; Enrico V. Avvedimento; Max E. Gottesman

doi:10.1016/S0014-5793(99)01585-9

Yotiao protein, a ligand for the NMDA receptor, binds and targets cAMP- dependent protein kinase II

Antonio Feliciello, Luca Cardone, Corrado Garbi, Michael D. Ginsberg, Stelio Varrone, Charles S. Rubin, Enrico V. Avvedimento, Max E. Gottesman

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

A yeast two-hybrid screen revealed that regulatory subunits (RII) of PKAII bind the Yotiao protein. Yotiao interacts with the NR1 subunit of the NMDA receptor. A purified C-terminal fragment of Yotiao binds PKAII, via an RII binding site constituted by amino acid residues 1452-1469, with a dissociation constant (K(d)) between 50 and 90 nM in vitro. A stable complex composed of Yotiao, RII and NR1 was immunoprecipitated from whole rat brain extracts. Immunostaining analysis disclosed that Yotiao, RIIβ and NR1 colocalize in striatal and cerebellar neurons. Co-assembly of Yotiao/PKAII complexes with NR1 subunits may promote cAMP-dependent modulation of NMDA receptor activity at synapses, thereby influencing brain development and synaptic plasticity.

Original language	English (US)
Pages (from-to)	174-178
Number of pages	5
Journal	FEBS Letters
Volume	464
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(99)01585-9
State	Published - Dec 31 1999
Externally published	Yes

Keywords

NMDA receptor
Protein kinase A
Yotiao protein

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(99)01585-9

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@article{81f44268d2c142d986cd8a704db785b9,

title = "Yotiao protein, a ligand for the NMDA receptor, binds and targets cAMP- dependent protein kinase II",

abstract = "A yeast two-hybrid screen revealed that regulatory subunits (RII) of PKAII bind the Yotiao protein. Yotiao interacts with the NR1 subunit of the NMDA receptor. A purified C-terminal fragment of Yotiao binds PKAII, via an RII binding site constituted by amino acid residues 1452-1469, with a dissociation constant (K(d)) between 50 and 90 nM in vitro. A stable complex composed of Yotiao, RII and NR1 was immunoprecipitated from whole rat brain extracts. Immunostaining analysis disclosed that Yotiao, RIIβ and NR1 colocalize in striatal and cerebellar neurons. Co-assembly of Yotiao/PKAII complexes with NR1 subunits may promote cAMP-dependent modulation of NMDA receptor activity at synapses, thereby influencing brain development and synaptic plasticity.",

keywords = "NMDA receptor, Protein kinase A, Yotiao protein",

author = "Antonio Feliciello and Luca Cardone and Corrado Garbi and Ginsberg, {Michael D.} and Stelio Varrone and Rubin, {Charles S.} and Avvedimento, {Enrico V.} and Gottesman, {Max E.}",

note = "Funding Information: This work was supported by grants from NIH grant DK50702 (M.E.G.); from Associazione Italiana Ricerca sul Cancro (A.I.R.C.), Progetti Finalizzati CNR {\textquoteleft}Biotecnologie{\textquoteright} Italy (E.V.A.); NIH grant GM 22792 (C.S.R.). A.F. was partly supported by a Fellowship from {\textquoteleft}International Agency for Research on Cancer World Health Organization (Lyon, France){\textquoteright}. We thank Dr. B.B. Wolphe from the Department of Pharmacology, Georgetown University, Washington, DC, for kindly providing NR1 monoclonal antibodies (grant NS28130); special thanks to Dr. M. Paolillo, University of Pavia, Italy, for providing primary cultures of striatal neurons.",

year = "1999",

month = dec,

day = "31",

doi = "10.1016/S0014-5793(99)01585-9",

language = "English (US)",

volume = "464",

pages = "174--178",

journal = "FEBS Letters",

issn = "0014-5793",

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TY - JOUR

T1 - Yotiao protein, a ligand for the NMDA receptor, binds and targets cAMP- dependent protein kinase II

AU - Feliciello, Antonio

AU - Cardone, Luca

AU - Garbi, Corrado

AU - Ginsberg, Michael D.

AU - Varrone, Stelio

AU - Rubin, Charles S.

AU - Avvedimento, Enrico V.

AU - Gottesman, Max E.

N1 - Funding Information: This work was supported by grants from NIH grant DK50702 (M.E.G.); from Associazione Italiana Ricerca sul Cancro (A.I.R.C.), Progetti Finalizzati CNR ‘Biotecnologie’ Italy (E.V.A.); NIH grant GM 22792 (C.S.R.). A.F. was partly supported by a Fellowship from ‘International Agency for Research on Cancer World Health Organization (Lyon, France)’. We thank Dr. B.B. Wolphe from the Department of Pharmacology, Georgetown University, Washington, DC, for kindly providing NR1 monoclonal antibodies (grant NS28130); special thanks to Dr. M. Paolillo, University of Pavia, Italy, for providing primary cultures of striatal neurons.

PY - 1999/12/31

Y1 - 1999/12/31

N2 - A yeast two-hybrid screen revealed that regulatory subunits (RII) of PKAII bind the Yotiao protein. Yotiao interacts with the NR1 subunit of the NMDA receptor. A purified C-terminal fragment of Yotiao binds PKAII, via an RII binding site constituted by amino acid residues 1452-1469, with a dissociation constant (K(d)) between 50 and 90 nM in vitro. A stable complex composed of Yotiao, RII and NR1 was immunoprecipitated from whole rat brain extracts. Immunostaining analysis disclosed that Yotiao, RIIβ and NR1 colocalize in striatal and cerebellar neurons. Co-assembly of Yotiao/PKAII complexes with NR1 subunits may promote cAMP-dependent modulation of NMDA receptor activity at synapses, thereby influencing brain development and synaptic plasticity.

AB - A yeast two-hybrid screen revealed that regulatory subunits (RII) of PKAII bind the Yotiao protein. Yotiao interacts with the NR1 subunit of the NMDA receptor. A purified C-terminal fragment of Yotiao binds PKAII, via an RII binding site constituted by amino acid residues 1452-1469, with a dissociation constant (K(d)) between 50 and 90 nM in vitro. A stable complex composed of Yotiao, RII and NR1 was immunoprecipitated from whole rat brain extracts. Immunostaining analysis disclosed that Yotiao, RIIβ and NR1 colocalize in striatal and cerebellar neurons. Co-assembly of Yotiao/PKAII complexes with NR1 subunits may promote cAMP-dependent modulation of NMDA receptor activity at synapses, thereby influencing brain development and synaptic plasticity.

KW - NMDA receptor

KW - Protein kinase A

KW - Yotiao protein

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JO - FEBS Letters

JF - FEBS Letters

IS - 3

ER -

Yotiao protein, a ligand for the NMDA receptor, binds and targets cAMP- dependent protein kinase II

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Keywords

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