Yotiao protein, a ligand for the NMDA receptor, binds and targets cAMP- dependent protein kinase II

Antonio Feliciello, Luca Cardone, Corrado Garbi, Michael D. Ginsberg, Stelio Varrone, Charles S. Rubin, Enrico V. Avvedimento, Max E. Gottesman

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

A yeast two-hybrid screen revealed that regulatory subunits (RII) of PKAII bind the Yotiao protein. Yotiao interacts with the NR1 subunit of the NMDA receptor. A purified C-terminal fragment of Yotiao binds PKAII, via an RII binding site constituted by amino acid residues 1452-1469, with a dissociation constant (K(d)) between 50 and 90 nM in vitro. A stable complex composed of Yotiao, RII and NR1 was immunoprecipitated from whole rat brain extracts. Immunostaining analysis disclosed that Yotiao, RIIβ and NR1 colocalize in striatal and cerebellar neurons. Co-assembly of Yotiao/PKAII complexes with NR1 subunits may promote cAMP-dependent modulation of NMDA receptor activity at synapses, thereby influencing brain development and synaptic plasticity.

Original languageEnglish (US)
Pages (from-to)174-178
Number of pages5
JournalFEBS Letters
Volume464
Issue number3
DOIs
StatePublished - Dec 31 1999
Externally publishedYes

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Cyclic AMP-Dependent Protein Kinases
N-Methyl-D-Aspartate Receptors
Brain
Ligands
Corpus Striatum
Neuronal Plasticity
Yeast
Synapses
Neurons
Plasticity
Rats
Proteins
Yeasts
Binding Sites
Modulation
Amino Acids
NR1 NMDA receptor
In Vitro Techniques

Keywords

  • NMDA receptor
  • Protein kinase A
  • Yotiao protein

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Feliciello, A., Cardone, L., Garbi, C., Ginsberg, M. D., Varrone, S., Rubin, C. S., ... Gottesman, M. E. (1999). Yotiao protein, a ligand for the NMDA receptor, binds and targets cAMP- dependent protein kinase II. FEBS Letters, 464(3), 174-178. https://doi.org/10.1016/S0014-5793(99)01585-9

Yotiao protein, a ligand for the NMDA receptor, binds and targets cAMP- dependent protein kinase II. / Feliciello, Antonio; Cardone, Luca; Garbi, Corrado; Ginsberg, Michael D.; Varrone, Stelio; Rubin, Charles S.; Avvedimento, Enrico V.; Gottesman, Max E.

In: FEBS Letters, Vol. 464, No. 3, 31.12.1999, p. 174-178.

Research output: Contribution to journalArticle

Feliciello, A, Cardone, L, Garbi, C, Ginsberg, MD, Varrone, S, Rubin, CS, Avvedimento, EV & Gottesman, ME 1999, 'Yotiao protein, a ligand for the NMDA receptor, binds and targets cAMP- dependent protein kinase II', FEBS Letters, vol. 464, no. 3, pp. 174-178. https://doi.org/10.1016/S0014-5793(99)01585-9
Feliciello A, Cardone L, Garbi C, Ginsberg MD, Varrone S, Rubin CS et al. Yotiao protein, a ligand for the NMDA receptor, binds and targets cAMP- dependent protein kinase II. FEBS Letters. 1999 Dec 31;464(3):174-178. https://doi.org/10.1016/S0014-5793(99)01585-9
Feliciello, Antonio ; Cardone, Luca ; Garbi, Corrado ; Ginsberg, Michael D. ; Varrone, Stelio ; Rubin, Charles S. ; Avvedimento, Enrico V. ; Gottesman, Max E. / Yotiao protein, a ligand for the NMDA receptor, binds and targets cAMP- dependent protein kinase II. In: FEBS Letters. 1999 ; Vol. 464, No. 3. pp. 174-178.
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