Yeast mitochondrial transcriptomics.

Mathilde Garcia, Xavier Darzacq, Frederic Devaux, Robert H. Singer, Claude Jacq

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Although 30 years ago it was strongly suggested that some cytoplasmic ribosomes are bound to the surface of yeast mitochondria, the mechanisms and the raison d'être of this process are not understood. For instance, it is not perfectly known which of the several hundred nuclearly encoded genes have to be translated to the mitochondrial vicinity to guide the import of the corresponding proteins. One can take advantage of several modern methods to address a number of aspects of the site-specific translation process of messenger ribonucleic acid (mRNA) coding for proteins imported into mitochondria. Three complementary approaches are presented to analyze the spatial distribution of mRNAs coding for proteins imported into mitochondria. Starting from biochemical purifications of mitochondria-bound polysomes, we describe a genomewide approach to classify all the cellular mRNAs according to their physical proximity with mitochondria; we also present real-time quantitative reverse transcription polymerase chain reaction monitoring of mRNA distribution to provide a quantified description of this localization. Finally, a fluorescence microscopy approach on a single living cell is described to visualize the in vivo localization of mRNAs involved in mitochondria biogenesis.

Original languageEnglish (US)
Pages (from-to)505-528
Number of pages24
JournalMethods in molecular biology (Clifton, N.J.)
Volume372
StatePublished - 2007
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Garcia, M., Darzacq, X., Devaux, F., Singer, R. H., & Jacq, C. (2007). Yeast mitochondrial transcriptomics. Methods in molecular biology (Clifton, N.J.), 372, 505-528.