Yeast and mammalian replication intermediates migrate similarly in two-dimensional gels

C. Brun, P. A. Dijkwel, R. D. Little, J. L. Hamlin, Carl L. Schildkraut, J. A. Huberman

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

In the budding yeast, Saccharomyces cerevisiae, DNA replication initiates at specific, discrete chromosomal locations. At each initiation site, a single small replication bubble is generated, which subsequently expands at Y-like replication forks. We wanted to know whether other eukaryotic organisms utilize similar initiation mechanisms. For this purpose, replication intermediates (RIs) from three different organisms (Schizosaccharomyces pombe, Chinese hamster and human) were mixed individually with RIs from S. cerevisiae and then subjected to two-dimensional (2D) gel electrophoresis under conditions known to resolve molecules having different structures. All of the RIs detected by the hybridization probes we used for each organism migrated nearly identically to specific RIs of similar size from S. cerevisiae, implying that the detected RIs from all the studied organisms have very similar structures and may therefore employ the same basic initiation mechanism.

Original languageEnglish (US)
Pages (from-to)92-102
Number of pages11
JournalChromosoma
Volume104
Issue number2
DOIs
StatePublished - 1995

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Saccharomyces cerevisiae
Yeasts
Gels
Electrophoresis, Gel, Two-Dimensional
Saccharomycetales
Schizosaccharomyces
Cricetulus
DNA Replication

ASJC Scopus subject areas

  • Genetics

Cite this

Yeast and mammalian replication intermediates migrate similarly in two-dimensional gels. / Brun, C.; Dijkwel, P. A.; Little, R. D.; Hamlin, J. L.; Schildkraut, Carl L.; Huberman, J. A.

In: Chromosoma, Vol. 104, No. 2, 1995, p. 92-102.

Research output: Contribution to journalArticle

Brun, C, Dijkwel, PA, Little, RD, Hamlin, JL, Schildkraut, CL & Huberman, JA 1995, 'Yeast and mammalian replication intermediates migrate similarly in two-dimensional gels', Chromosoma, vol. 104, no. 2, pp. 92-102. https://doi.org/10.1007/s004120050096
Brun, C. ; Dijkwel, P. A. ; Little, R. D. ; Hamlin, J. L. ; Schildkraut, Carl L. ; Huberman, J. A. / Yeast and mammalian replication intermediates migrate similarly in two-dimensional gels. In: Chromosoma. 1995 ; Vol. 104, No. 2. pp. 92-102.
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