X-linked control of hemoglobin production in somatic hybrids of mouse erythroleukemic cells and mouse lymphoma or bone marrow cells

Susan Benoff, Arthur I. Skoultchi

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Somatic cell hybrids were generated by fusion of mouse erythroleukemic cells (clone 745) to mouse lymphoma cells or mouse bone marrow cells. The erythroleukemic cells have been shown previously to have a low basal level of erythroid differentiation which is markedly amplified when the cells are grown in medium containing dimethylsulfoxide (DMSO). Hybrid cells were examined for hemoglobin production by benzidine staining. Many hybrid clones were found in which hemoglobin production in response to DMSO was either abolished or greatly reduced. From these hybrids, subclones were isolated in which hemoglobin production was restored. Karyological and enzyme analysis showed that the restoration of hemoglobin production was associated with the loss of an X chromosome contributed by the nonerythroleukemic parent. Other subclones which retained an X chromosome continued to be inhibited for hemoglobin production. Analysis of other hybrid lines capable of a limited degree of erythroid differentiation indicated a quantitative inverse correlation between the proportion of cells bearing an X chromosome and the proportion of cells able to form hemoglobin. Finally, four hybrid lines having many cells without an X chromosome were grown in medium containing DMSO. This procedure led to the selection of hybrid sublines having a nondifferentiating phenotype and concomitantly having a greatly increased proportion of cells bearing an X chromosome. Thus three lines of evidence suggest that a locus (loci) on the X chromosome is capable of inhibiting the DMSO-inducible hemoglobin production of the erythroleukemic cells.

Original languageEnglish (US)
Pages (from-to)263-274
Number of pages12
JournalCell
Volume12
Issue number1
DOIs
StatePublished - 1977

Fingerprint

Bone Marrow Cells
Chromosomes
Lymphoma
Bone
Hemoglobins
X Chromosome
Cells
Dimethyl Sulfoxide
Bearings (structural)
Hybrid Cells
Clone cells
Clone Cells
Restoration
Fusion reactions
Staining and Labeling
Phenotype
Enzymes

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

X-linked control of hemoglobin production in somatic hybrids of mouse erythroleukemic cells and mouse lymphoma or bone marrow cells. / Benoff, Susan; Skoultchi, Arthur I.

In: Cell, Vol. 12, No. 1, 1977, p. 263-274.

Research output: Contribution to journalArticle

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